Detects human CCL15/MIP‑1δ in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human
CCL1, 2, 3, 4, 5, 7, 8, 11, 13, 14, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26,
recombinant mouse CCL1, 2, 3, 4, 5, 6, 7, 9, 11, 12, 17, 19, 20, 21, 22, 24,
25, or recombinant rat CCL20 is observed.
Monoclonal Mouse IgG1 Clone # 88119
Protein A or G purified from ascites
E. coli-derived recombinant human CCL15/MIP-1δ Ser46-Ile113 Accession # Q16663
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.10 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CCL15/MIP‑1δ 92 aa (Catalog # 363-MG)
Measured by its ability to neutralize CCL15/MIP‑1δ-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 5-20 µg/mL in the presence of 1 ng/mL Recombinant Human CCL15/MIP‑1δ 68 aa.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL15/MIP‑1δ and Neutralization by Human CCL15/ MIP‑1δ Antibody. Recombinant Human CCL15/ MIP‑1δ (Catalog # 628-LK) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP‑1δ (1 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human CCL15/MIP‑1δ Monoclonal Antibody (Catalog # MAB3631). The ND50 is typically 5-20 µg/mL.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL15/MIP-1 delta
CCL15, also called macrophage inflammatory protein 1 delta (MIP-1δ and Leukotactin-1 (LKN-1), is a CC chemokine with two additional cysteine residues that form a third disulfide bond. CCL15 functions as a chemoattractant for monocytes, T cells, and eosinophils through interactions with CCR1. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells.
Wang, W. et al. (1998) J. Clinical Immunol. 18:214.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.