Chemotaxis Induced by CCL15/MIP‑1δ and Neutralization by Human CCL15/|
Recombinant Human CCL15/
MIP‑1δ (Catalog # 628-LK) chemoattracts the THP‑1 human acute monocytic leukemia cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Human CCL15/
MIP‑1δ (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti‑Human CCL15/MIP‑1δ Monoclonal Antibody (Catalog # MAB3631). The ND50 is typically 5-20 µg/mL.
CCL15, also called macrophage inflammatory protein 1 delta (MIP-1δ and Leukotactin-1 (LKN-1), is a CC chemokine with two additional cysteine residues that form a third disulfide bond. CCL15 functions as a chemoattractant for monocytes, T cells, and eosinophils through interactions with CCR1. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1δ is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells.
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