Human CCL23/Ck beta 8-1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects CCL23/Ck beta 8‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human MPIF-1 is observed. Additionally, in direct ELISAs, no cross-reactivity with _______ is observed. Neutralizes the biological activity of human CCL23/Ck beta 8‑1. It will also neutralize the biological activity of recombinant human MPIF-1 at a 10-fold higher Ig concentration.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human CCL23/Ck beta 8‑1
    Met46-Asn137
    Accession # AAD10846
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.20 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human CCL23/Ck beta 8‑1 aa 46-137 (Catalog # 508-CK)
  • Neutralization
    Measured by its ability to neutralize CCL23-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL23/MPIF‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Chemotaxis Induced by CCL23 and Neutralization by Human CCL23 Antibody. Recombinant Human CCL23/
MPIF‑1 (Catalog # 371‑MP) chemo­attracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/
MPIF‑1 (0.04 µg/mL) is neutral­ized (green line) by increasing concentrations of Goat Anti-Human CCL23/Ck beta 8‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF508). The ND50 is typically 0.15‑0.75 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL23/Ck beta 8-1
Ck beta 8-1 (SCYA23), a splice variant of Ck beta 8 (also known as MPIF-1), is a CC chemokine. Compared to Ck beta 8-1, Ck beta 8/MPIF-1 lacks a 17 amino acid residue stretch (Leu47-Gly 63) present in Ck beta 8-1 and has a unique arginine at residue 46. Ck beta 8-1/Ck beta 8 (MPIF-1) and leukotactin/MIP-1 delta form a subgroup of CC chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. Ck beta 8-1 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1 delta).
  • Entrez Gene IDs:
    6368 (Human)
  • Alternate Names:
    C6 beta-chemokine; CCL23/Ck beta 8-1; chemokine (C-C motif) ligand 23; Ck beta 8-1; CKb8; Ckb-8; Ckb81; CK-beta-8; hmrp-2a; Macrophage inflammatory protein 3; MIP3; MIP-3CK-BETA-8; MPIF1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; SCYA23C-C motif chemokine 23; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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