Detects CCL23/Ck beta 8‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human MPIF-1 is observed. Additionally, in direct ELISAs, no cross-reactivity with _______ is observed. Neutralizes the biological activity of human CCL23/Ck beta 8‑1. It will also neutralize the biological activity of recombinant human MPIF-1 at a 10-fold higher Ig concentration.
Polyclonal Goat IgG
E. coli-derived recombinant human CCL23/Ck beta 8‑1 Met46-Asn137 Accession # AAD10846
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
<0.20 EU per 1 μg of the antibody by the LAL method.
Recombinant Human CCL23/Ck beta 8‑1 aa 46-137 (Catalog # 508-CK)
Measured by its ability to neutralize CCL23-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 0.04 µg/mL Recombinant Human CCL23/MPIF‑1.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Chemotaxis Induced by CCL23 and Neutralization by Human CCL23 Antibody. Recombinant Human CCL23/ MPIF‑1 (Catalog # 371‑MP) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL23/ MPIF‑1 (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL23/Ck beta 8‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF508). The ND50 is typically 0.15‑0.75 µg/mL.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL23/Ck beta 8-1
Ck beta 8-1 (SCYA23), a splice variant of Ck beta 8 (also known as MPIF-1), is a CC chemokine. Compared to Ck beta 8-1, Ck beta 8/MPIF-1 lacks a 17 amino acid residue stretch (Leu47-Gly 63) present in Ck beta 8-1 and has a unique arginine at residue 46. Ck beta 8-1/Ck beta 8 (MPIF-1) and leukotactin/MIP-1 delta form a subgroup of CC chemokines that have 6 conserved cysteine residues and an extended amino-terminus preceding the conserved cysteine pair. Ck beta 8-1 shares approximately 73% nucleotide sequence identity with leukotactin (MIP-1 delta).
Entrez Gene IDs:
C6 beta-chemokine; chemokine (C-C motif) ligand 23; Ck beta 8-1; CKb8; Ckb-8; Ckb81; CK-beta-8; hmrp-2a; Macrophage inflammatory protein 3; MIP3; MIP-3CK-BETA-8; MPIF1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; SCYA23C-C motif chemokine 23; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23
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