Human CCL5/RANTES Luminex Performance Assay

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Citations (13)
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Human CCL5/RANTES Luminex Performance Assay Summary

Panel Luminex Performance Human Cytokine Panel A
Assay Type Polystyrene bead-based multiplex assay for the Luminex® platform
Format 1 x 96-well microplate and Polystyrene antibody-coated beads
Species Human
Analytes Detected Please see analyte list in assay customization tool below.
Performance Validation Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

Make your selections below to configure your assay

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How would you like these analytes mixed?

Premixed
User Mixed
Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

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Split based on serum
Split based on plasma
Split based on cell culture supernates
Information for Compatible Analytes

For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services.

Product Features

Polystyrene Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

  • Simultaneously profile Human CCL5/RANTES with up to analytes of your choice in one sample
  • Undergoes similar validation testing as Quantikine® ELISA Kits
  • Precise and Specific
  • Requires a small sample volume (<50 µL)
  • Run your assay in just 3-5 hours
  • Mass-calibrated standards for consistent results with every new lot of material
  • Enough reagents for one 96-well plate
Base Kit Components
  • Standard Cocktail(s)
  • Bead Diluent
  • Biotin Antibody Diluent
  • Calibrator Diluent
  • Wash Buffer
  • Streptavidin-PE
  • One filter-bottom 96-well Microplate
  • Foil Plate Sealers (4)
  • Mixing Bottle
  • Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit.

Bead Set Contents
  • User-mix antibody-coated Polystyrene beads in individual vials
  • User-mixed cocktail of biotinylated detection antibody
Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Polystyrene Bead-based Multiplex Kits For The Luminex® Platform

Polystyrene Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or Bio-Rad® Bio-Plex®, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaPolystyrene microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.

Product Datasheets

Citations for Human CCL5/RANTES Luminex Performance Assay

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. N6-isopentenyladenosine, an endogenous isoprenoid end product, directly affects cytotoxic and regulatory functions of human NK cells through FDPS modulation.
    Authors: Ciaglia E, Pisanti S, Picardi P, Laezza C, Malfitano A, D'Alessandro A, Gazzerro P, Vitale M, Carbone E, Bifulco M
    J Leukoc Biol, 2013;94(6):1207-19.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
    J. Thromb. Haemost., 2012;10(8):1616-23.
    Species: Human
    Sample Types: Plasma
  3. Elevated cerebral spinal fluid cytokine levels in boys with cerebral adrenoleukodystrophy correlates with MRI severity.
    Authors: Lund TC, Stadem PS, Panoskaltsis-Mortari A, Raymond G, Miller WP, Tolar J, Orchard PJ
    PLoS ONE, 2012;7(2):e32218.
    Species: Human
    Sample Types: Serum
  4. Potential biomarkers of osteonecrosis in Gaucher disease.
    Authors: Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM
    Blood Cells Mol. Dis., 2011;46(1):27-33.
    Species: Human
    Sample Types: Serum
  5. Macrophage infiltration predicts a poor prognosis for human ewing sarcoma.
    Authors: Fujiwara T, Fukushi J, Yamamoto S, Matsumoto Y, Setsu N, Oda Y, Yamada H, Okada S, Watari K, Ono M, Kuwano M, Kamura S, Iida K, Okada Y, Koga M, Iwamoto Y
    Am. J. Pathol., 2011;179(3):1157-70.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Neisseria gonorrhoeae activates the proteinase cathepsin B to mediate the signaling activities of the NLRP3 and ASC-containing inflammasome.
    Authors: Duncan JA, Gao X, Huang MT, O'Connor BP, Thomas CE, Willingham SB, Bergstralh DT, Jarvis GA, Sparling PF, Ting JP
    J. Immunol., 2009;182(10):6460-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Scaleable manufacture of HIV-1 entry inhibitor griffithsin and validation of its safety and efficacy as a topical microbicide component.
    Authors: O'Keefe BR, Vojdani F, Buffa V, Shattock RJ, Montefiori DC, Bakke J, Mirsalis J, d'Andrea AL, Hume SD, Bratcher B, Saucedo CJ, McMahon JB, Pogue GP, Palmer KE
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(15):6099-104.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. Endothelial dysfunction and specific inflammation in obesity hypoventilation syndrome.
    Authors: Borel JC, Roux-Lombard P, Tamisier R, Arnaud C, Monneret D, Arnol N, Baguet JP, Levy P, Pepin JL
    PLoS ONE, 2009;4(8):e6733.
    Species: Human
    Sample Types: Serum
  9. Critical and differential roles of NKp46- and NKp30-activating receptors expressed by uterine NK cells in early pregnancy.
    Authors: El Costa H, Casemayou A, Aguerre-Girr M, Rabot M, Berrebi A, Parant O, Clouet-Delannoy M, Lombardelli L, Jabrane-Ferrat N, Rukavina D, Bensussan A, Piccinni MP, Le Bouteiller P, Tabiasco J
    J. Immunol., 2008;181(0):3009-17.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. Circulating chemokine levels and miscarriage.
    Authors: Whitcomb BW, Schisterman EF, Klebanoff MA, Baumgarten M, Rhoton-Vlasak A, Luo X, Chegini N
    Am. J. Epidemiol., 2007;166(3):323-31.
    Species: Human
    Sample Types: Serum
  11. Sensitization of IL-2 signaling through TLR-7 enhances B lymphoma cell immunogenicity.
    Authors: Tomic J, White D, Shi Y, Mena J, Hammond C, He L, Miller RL, Spaner DE
    J. Immunol., 2006;176(6):3830-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Coengagement of CD16 and CD94 receptors mediates secretion of chemokines and induces apoptotic death of naive natural killer cells.
    Authors: Jewett A, Cacalano NA, Head C, Teruel A
    Clin. Cancer Res., 2006;12(7):1994-2003.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Biological material on inhaled coarse fraction particulate matter activates airway phagocytes in vivo in healthy volunteers.
    Authors: Alexis NE, Lay JC, Zeman K, Bennett WE, Peden DB, Soukup JM, Devlin RB, Becker S
    J. Allergy Clin. Immunol., 2006;117(6):1396-403.
    Species: Human
    Sample Types: Saliva

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