Human CD14 Antibody

(3 citations)
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human CD14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse CD14 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 134603
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human CD14
    Thr20-Cys352
    Accession # P08571
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human CD14 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 5 µg/mL Human CD14 Monoclonal Antibody (Catalog # MAB3831) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human CD14 (Catalog # 383-CD) (5 ng) was included. A specific band for CD14 was detected at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD14

CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) precursor protein with a 19 aa signal peptide and a C‑terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14.

  • References:
    1. Wright, S.D. et al. (1990) Science 249:1431.
    2. Pugin, J. et al. (1993) Proc. Natl. Acad. Sci. USA 90:2744.
    3. Beutler, B. (2000) Current Opinion in Immunology 12:20.
    4. Stelter, F. (2000) Chem. Immunol. 74:25.
  • Entrez Gene IDs:
    929 (Human); 12475 (Mouse); 60350 (Rat); 100037938 (Porcine)
  • Alternate Names:
    CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Species
Applications
Sample Type
  1. 1,25-Dihydroxyvitamin D3 promotes a sustained LPS-induced NF-kappaB-dependent expression of CD55 in human monocytic THP-1 cells.
    Authors: Izban M, Nowicki B, Nowicki S
    PLoS ONE, 2012;7(11):e49318.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Soluble forms of Toll-like receptor 4 are present in human saliva and modulate tumour necrosis factor-alpha secretion by macrophage-like cells.
    Authors: Zunt SL, Burton LV, Goldblatt LI, Dobbins EE, Srinivasan M
    Clin. Exp. Immunol., 2009;156(2):285-93.
    Species: Human
    Sample Type: Saliva
    Application: Immunodepletion
  3. Soluble CD14 and toll-like receptor-2 are potential salivary biomarkers for oral lichen planus and burning mouth syndrome.
    Authors: Srinivasan M, Kodumudi KN, Zunt SL
    Clin. Immunol., 2007;126(1):31-7.
    Species: Human
    Sample Type: Saliva
    Application: WB
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Isotype Control

Ctrl MAB002 138  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG1 Isotype Control

Ctrl MAB002R 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG Antibody

WB AF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Average Rating: 4 (Based on 1 review)

Have you used Human CD14 Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Read what people are saying who have used Human CD14 Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

Filter by Ratings
Very Good
(1)
All Ratings
Filter by Applications
IHC
(1)
All Applications
Filter by Species
Human
(1)
All Species

Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human CD14 Antibody MAB3831
Enlarge
Very Good
 IHC Human Anonymous 05/03/2017
Show
Image Details
Immunohistochemistry Human CD14 Antibody MAB3831
Immunohistochemistry: Human CD14 Antibody [MAB3831]

Summary

ApplicationImmunohistochemistry
Sample TestedAdult lung
SpeciesHuman

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information