Protein A or G purified from hybridoma culture supernatant
Human embryonic kidney cell line HEK-293-derived human CD28H/IGPR-1 Met1-Gly150 Accession # Q96BF3
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
0.25 µg/106 cells
Blockade of Receptor-ligand Interaction
In a functionalflow cytometry test, 50 μg/mL of Mouse anti-Human CD28H/IGPR-1 Antibody(Catalog #MAB83162) will block the binding of 500 ng/mL Recombinant Human B7-H7Fc Chimera (Catalog #156-B7)to HEK293 human embryonic kidney cell line transfected with recombinant human CD28H.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CD28H/IGPR‑1 in HEK293 Human Cell Line Transfected with Human CD28H/IGPR-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human CD28H/IGPR-1 and eGFP was stained with and either (A) Mouse Anti-Human CD28H/IGPR‑1 Monoclonal Antibody (Catalog # MAB83162) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
B7-H7Binding to CD28H-transfected HEK293 Human Cell Line is Blocked byHuman CD28H Antibody. In a functional flow cytometry test,Recombinant Human B7-H7 Fc Chimera (Catalog #156-B7, 500 ng/mL) binds to HEK293 human embryonic kidney cell linetransfected with recombinant human CD28H (black dotted line). Binding iscompletely blocked (orange histogram) by 50 μg/mL of Mouse Anti-Human CD28H/IGPR-1Monoclonal Antibody (Catalog # MAB83162). Mouse IgG2B Isotype Control(Catalog #MAB003)at 50 μg/mL was used as a control (blue line). Cells were stained with MouseAnti-Human IgG1 Fc APC‑conjugated Monoclonal Antibody (Catalog #FAB110A).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells (1, 2). CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract (3). It regulates cellular morphology, focal adhesion contact formation, and cell migration (3, 4). CD28H also participates in angiogenesis in vitro (3). CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion (3, 5).
Zhu, Y. et al. (2013) Nat. Commun. 4:2043.
Parry, R.V. et al. (2003) J.Immunol. 171:166.
Rahimi, N. et al. (2012) Mol. Biol. Cell. 23:1646.
Huang, C. et al. (2003) Nature 424:219.
Kaneko, T. et al. (2008) Front. Biosci. 13:4938.
Transmembrane And Immunoglobulin Domain Containing 2