Human CD44v3 PE‑conjugated Antibody

FAB5088P has been discontinued. View all CD44 products.
(5 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human CD44v3 in Western blots. The specificity of monoclonal antibody clone 3G5 was determined by FACS analysis on a panel of CD44 transfected COS cells, and was deduced to be specific for CD44 protein isoforms containing human variant exon 3 (9).
  • Source
    Monoclonal Mouse IgG2B Clone # 3G5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Recombinant human CD44v3-10
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD44v3 in MDA‑MB‑231 Human breast cancer Cell Line by Flow Cytometry. MDA‑MB‑231 human breast cancer cell line was stained with Mouse Anti-Human CD44v3 PE‑conjugated Monoclonal Antibody (Catalog # FAB5088P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD44

CD44 is a ubiquitously expressed protein that is the major receptor for hyaluronan and exerts control over cell growth and migration (1-3). Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. Within the stem, ten variably spliced exons (v1-10, exons 6-15) produce multiple protein isoforms (1‑3). The standard or hematopoietic form, CD44H, does not include the variable segments (1-3). Cancer aggressiveness and T cell activation have been correlated with expression of specific isoforms (1, 3). With variable N- and O-glycosylation and splicing within the stalk, CD44 can range from 80 to 200 kDa (1). Within the N‑terminal invariant portion of the ECD (aa 21-220), human CD44 shares 76%, 76%, 86%, 83% and 79% aa sequence identity with corresponding mouse, rat, equine, canine and bovine CD44, respectively. The many reported functions of CD44 fall within three categories (1). First, CD44 binds hyaluronan and other ligands within the extracellular matrix and can function as a “platform” for growth factors and metalloproteinases. Second, CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Third, the CD44 intracellular domain links the plasma membrane to the actin cytoskeleton via the ERM proteins, Ezrin, Radixin and Moesin. CD44 can be synthesized in a soluble form (4) or may be cleaved at multiple sites by either membrane-type matrix metalloproteinases, or ADAM proteases to produce soluble ectodomains (5, 6). The cellular portion may then undergo gamma secretase-dependent intramembrane cleavage to form an A beta -like transmembrane portion and a cytoplasmic signaling portion that affects gene expression (7, 8). These cleavage events are thought to promote metastasis by enhancing tumor cell motility and growth (1, 5).

  • References:
    1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
    2. Screaton, G.R. et al. (1992) Proc. Natl. Acad. Sci. USA 89:12160.
    3. Lynch, K.W. (2004) Nat. Rev. Immunol. 4:931.
    4. Yu, Q. and B.P. Toole (1996) J. Biol. Chem. 271:20603.
    5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
    6. Nakamura, H. et al. (2004) Cancer Res. 64:876.
    7. Murakami, D. et al. (2003) Oncogene 22:1511.
    8. Lammich, S. et al. (2002) J. Biol. Chem. 277:44754.
    9. Fox, S.B. et al. (1994) Cancer Res. 54:4539.
  • Entrez Gene IDs:
    960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)
  • Alternate Names:
    CD44 antigen; CD44 molecule (Indian blood group); CD44; CD44R; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; CSPG8; ECMR-III; epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; HCAM; HCELL; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; Hyaluronate receptor; IN; LHR; MC56; MDU2; MDU2CD44 antigen (homing function and Indian blood group system); MDU3; MDU3CDW44; MIC4; MIC4MGC10468; MUTCH-I; Pgp1; PGP-1; PGP-I; Phagocytic glycoprotein 1; Phagocytic glycoprotein I
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. Coexpression of EpCAM, CD44 variant isoforms and claudin-7 in anaplastic thyroid carcinoma.
    Authors: Okada, Toshihir, Nakamura, Teruo, Watanabe, Takayuki, Onoda, Naoyoshi, Ashida, Atsuko, Okuyama, Ryuhei, Ito, Ken-ichi
    PLoS ONE, 2014;9(4):e94487.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Evaluation of cancer stem cell markers CD133, CD44, CD24: association with AKT isoforms and radiation resistance in colon cancer cells.
    Authors: Sahlberg S, Spiegelberg D, Glimelius B, Stenerlow B, Nestor M
    PLoS ONE, 2014;9(4):e94621.
  3. CD44 staining of cancer stem-like cells is influenced by down-regulation of CD44 variant isoforms and up-regulation of the standard CD44 isoform in the population of cells that have undergone epithelial-to-mesenchymal transition.
    Authors: Biddle, Adrian, Gammon, Luke, Fazil, Bilal, Mackenzie, Ian C
    PLoS ONE, 2013;8(2):e57314.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Maintenance of stem cell self-renewal in head and neck cancers requires actions of GSK3beta influenced by CD44 and RHAMM.
    Authors: Shigeishi H, Biddle A, Gammon L, Emich H, Rodini C, Gemenetzidis E, Fazil B, Sugiyama M, Kamata N, Mackenzie I
    Stem Cells, 2013;31(10):2073-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. DcR3 binds to ovarian cancer via heparan sulfate proteoglycans and modulates tumor cells response to platinum with corresponding alteration in the expression of BRCA1.
    BMC Cancer, 2012;12(0):176.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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