Human CD59 MAb (Clone 2491C)

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1987-100
MAB1987-SP
Detection of CD59 in Human PBMC lymphocytes by Flow Cytometry.
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Human CD59 MAb (Clone 2491C) Summary

Species Reactivity
Human
Specificity
Detects human CD59 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2491C
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Mouse myeloma cell line, NS0-derived recombinant human CD59
Leu26-Asn102
Accession # P13987
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CD59 in Human PBMC lymphocytes by Flow Cytometry. View Larger

Detection of CD59 in Human PBMC lymphocytes by Flow Cytometry. Human PBMC lymphocytes were stained with Rabbit Anti-Human CD59 Monoclonal Antibody (Catalog # MAB1987, filled histogram) or isotype control antibody (Catalog # MAB1050, open histogram) followed by anti-Rabbit IgG PE-conjugated secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD59

CD59, also known as membrane attack complex inhibition factor (MACIF) and Protectin, is an approximately 20 kDa GPI‑anchored glycoprotein that is an important regulator of the complement system in blood. The complement system triggers innate immune responses to immune complexes, MBL‑opsonized microorganisms, and apoptotic cells through the classical, lectin, and alternative pathways. One major consequence of complement activation is the assembly of a membrane attack complex (MAC) composed of one molecule each of complement proteins C5b, C6, C7, and C8 (C5b‑8) followed by the incorporation of multiple copies of C9 (C5b‑9). Membrane insertion of the MAC results in formation of a cytolytic pore in the target cell (1). CD59, which is widely expressed on healthy cells, binds to both C8 and C9 and shields them from complement‑mediated lysis. It inhibits MAC pore formation by blocking C5b‑8 complex membrane insertion and the incorporation of C9 molecules (2‑4). The binding of CD59 to C8 and C9 is species‑selective, and this contributes to the restricted ability of MACs to lyse cells of other species (5). The cytoprotective function of CD59 plays a variety of roles in pathology. It limits tissue damage and inflammation following ischemia/reperfusion injury (6, 7). It also protects against the development of atherosclerosis and abdominal aortic aneurysms (8, 9). Its protectiveness can be inactivated by diabetes‑induced glycation, leading to increased MAC deposition and hemolytic anemia (10). In contrast, CD59 can be exploited to promote red cell lysis; it functions as a cellular receptor for the bacterial pore‑forming toxin Intermedilysin (11). CD59 can be incorporated into several enveloped viruses such as hepatitis C virus where it limits the destruction of virus particles (12). Aside from its complement regulatory functions, CD59 limits the activation of T cells following their interaction with antigen presenting cells (13), but it promotes NK cell activation through association with NKp30 and NKp46 (14). In mouse, gene duplication has given rise to two related proteins, CD59a and CD59b. Mature human CD59 shares 37%, 43%, and 44% amino acid sequence identity with mouse CD59a, mouse CD59b, and rat CD59, respectively (15).

References
  1. Ricklin, D. et al. (2010) Nat. Rev. Immunol. 11:785.
  2. Farkas, I. et al. (2002) J. Physiol. 539:537.
  3. Meri, S. et al. (1990) Immunology 71:1.
  4. Rollins, S.A. and P.J. Sims (1990) J. Immunol. 144:3478.
  5. Rollins, S.A. et al. (1991) J. Immunol. 146:2345.
  6. Turnberg, D. et al. (2004) Am. J. Physiol. 165:825.
  7. Zhang, J. et al. (2011) Am. J. Pathol. 179:2876.
  8. Wu, G. et al. (2009) Circ. Res. 104:550.
  9. Wu, G. et al. (2010) Circulation 121:1338.
  10. Acosta, J. et al. (2000) Proc. Natl. Acad. Sci. USA 97:5450.
  11. Giddings, K.S. et al. (2004) Nat. Str. Mol. Biol. 11:1173.
  12. Amet, T. et al. (2012) Hepatology 55:354.
  13. Xie, X.-H. et al. (2012) Cell. Immunol. 274:1.
  14. Marcenaro, E. et al. (2003) Eur. J. Immunol. 33:3367.
  15. Sugita, Y. et al. (1989) J. Biochem. 106:555.
Entrez Gene IDs
966 (Human)
Alternate Names
16.3A5; 1F5 antigen; 1F5; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16; CD59 antigen; CD59 antigen, complement regulatory protein; CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59; EJ16; EJ30; EJ30, EL32 and G344); EL32; FLJ38134; FLJ92039; G344; HRF20; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MACIF; MAC-inhibitory protein; MAC-IP; MEM43 antigen; MEM43; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MGC2354; MIC11; MIC11MSK21; MIN1; MIN2; MIN3; MIRL; p18-20; Protectin; surface anitgen recognized by monoclonal 16.3A5; T cell-activating protein

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