Human CD82/Kai-1 APC-conjugated Antibody

FAB4616A Datasheet / COA / SDS

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FAB4616A

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Detection of CD82/Kai‑1 in Human Blood Lymphocytes by Flow Cytometry.

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Human CD82/Kai-1 APC-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD82/Kai‑1. Stains human CD82/Kai‑1 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 423524

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD82/Kai‑1
Met1-Tyr267
Accession # AAH01821

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CD82/Kai‑1 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD82/Kai‑1 APC‑conjugated Monoclonal Antibody (Catalog # FAB4616A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD82/Kai-1

CD82, also known as KAI1, C33 and TSPAN27, is a widely expressed palmitoylated molecule in the tetraspanin superfamily. CD82 is synthesized as a 28 kDa core protein with mature molecular weights ranging up to 70 kDa due to variable glycosylation. CD82 functions as a suppressor of metastasis and is down‑regulated in many malignant tumors. It associates with IGSF8, DARC, the ganglioside GM2, and the HTLV-1 Gag protein. CD82 inhibits cell migration and metastasis by preventing integrin maturation and blocking integrin interactions with Met. Human CD82 shares 76% aa sequence identity with mouse and rat CD82.

Entrez Gene IDs

3732 (Human); 12521 (Mouse); 83628 (Rat)

Alternate Names

CD82 antigenC33; CD82 molecule; CD82; IA4; IA4C33 antigen; Inducible membrane protein R2; KAI1; KAI1GR15; kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyteantigen, antigen detected by monoclonal and IA4)); Metastasis suppressor Kangai-1; R2; SAR2; SAR2tetraspanin-27; ST6; ST6tspan-27; Suppressor of tumorigenicity 6 protein; Tetraspanin-27; TSPAN27; Tspan-27; TSPAN274F9