Human CEACAM-8/CD66b APC-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
FAB4246A-025
Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry.
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Human CEACAM-8/CD66b APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Stains human CEACAM‑8/CD66b transfectants but not irrelevant transfectants in flow cytometry.
Source
Recombinant Monoclonal Mouse IgG1 Clone # 913542
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant human CEACAM-8/CD66b
Gln35-His141
Accession # P31997
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry. View Larger

Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM‑8/CD66b APC‑conjugated Monoclonal Antibody (Catalog # FAB4246A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CEACAM‑8/CD66b in HEK293 Human Cell Line Transfected with Human CEACAM-8/CD66b and eGFP by Flow Cytometry. View Larger

Detection of CEACAM‑8/CD66b in HEK293 Human Cell Line Transfected with Human CEACAM-8/CD66b and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line either (A) transfected with human CEACAM-8/CD66b or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM‑8/CD66b APC‑conjugated Monoclonal Antibody (Catalog # FAB4246A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CEACAM-8/CD66b

CEACAM-8 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 8) also known as CD66b, CD67 and NCA-95, is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.

Long Name
Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
Entrez Gene IDs
1088 (Human)
Alternate Names
Carcinoembryonic antigen CGM6; carcinoembryonic antigen gene family member 6; carcinoembryonic antigen-related cell adhesion molecule 8; CD66b antigen; CD66b; CD67 antigen; CD67; CEACAM8; CEACAM-8; CGM6; NCA-95; Non-specific cross-reacting antigen NCA-95

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