Detection of CEACAM‑8/CD66b in Human Blood Granulocytes by Flow Cytometry.
Human peripheral blood granulocytes were stained with Mouse Anti-Human CEACAM‑8/CD66b APC‑conjugated Monoclonal Antibody (Catalog # FAB4246A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CEACAM‑8/CD66b in HEK293 Human Cell Line Transfected with Human CEACAM-8/CD66b and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line either (A) transfected with human CEACAM-8/CD66b or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human CEACAM‑8/CD66b APC‑conjugated Monoclonal Antibody (Catalog # FAB4246A). Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CEACAM-8 (Carcinoembryonic Antigen-related Cell Adhesion Molecule 8) also known as CD66b, CD67 and NCA-95, is a 90 kDa member of the CEACAM subfamily of the CEA family of proteins. It is expressed by neutrophils and eosinophils, and serves as a binding partner for CEACAM-6 and Galectin-3. Mature human CEACAM-8 is a 287 amino acid GPI-linked glycoprotein. It contains one V-type and two C2-type Ig-like domains. No definitive rodent CEACAM-8 has been reported.
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