Human CLEC10A/CD301 PE-conjugated Antibody

Discontinued Product

FAB48881P has been discontinued.
Detection of CLEC10A/CD301 in Human Dendritic Cells by Flow Cytometry.
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Human CLEC10A/CD301 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CLEC10A/CD301 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ASGPR1 or recombinant mouse MGL1 is observed.
Source
Monoclonal Mouse IgG2B Clone # 744812
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CLEC10A/CD301
Gln61-His316
Accession # Q8IUN9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CLEC10A/CD301 in Human Dendritic Cells by Flow Cytometry. View Larger

Detection of CLEC10A/CD301 in Human Dendritic Cells by Flow Cytometry. Human immature dendritic cells were stained with Mouse Anti-Human CLEC10A/CD301 PE‑conjugated Monoclonal Antibody (Catalog # FAB48881P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CLEC10A/CD301

CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64%-70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3-7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2-4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).

References
  1. Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
  2. Suzuki, N. et al. (1996) J. Immunol. 156:128. 
  3. Higashi, N. et al. (2002) J. Biol. Chem. 277:20686. 
  4. Valladeau, J. et al. (2001) J. Immunol. 167:5767.
  5. van Vliet, S.J. et al. (2006) Immunobiology 211:577. 
  6. van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
  7. Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
  8. van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
  9. Higashi, N. et al. (2002) Int. Immunol. 14:545.
  10. Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
  11. Takada, A. et al. (2004) J. Virol. 78:2943.
Long Name
Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1
Entrez Gene IDs
10462 (Human)
Alternate Names
CD301 antigen; CD301; CD301C-type lectin domain family 10 member A; CLEC10A; CLECSF13; CLECSF14; CLECSF14macrophage C-type lectin; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 14 (macrophage-derived); C-type lectin domain family 10, member A; C-type lectin superfamily member 14; HML2; HMLC-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 13 (macrophage-derived); macrophage lectin 2 (calcium dependent); Macrophage lectin 2; MGL

Product Datasheets

Citation for Human CLEC10A/CD301 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Phenotypic and functional consequences of different isolation protocols on skin mononuclear phagocytes
    Authors: RA Botting, KM Bertram, H Baharlou, KJ Sandgren, J Fletcher, JW Rhodes, H Rana, TM Plasto, XM Wang, JJ Lim, L Barnouti, MP Kohout, T Papadopoul, S Merten, N Olbourne, AL Cunningham, M Haniffa, AN Harman
    J. Leukoc. Biol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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