Human Common gamma Chain/IL-2 R gamma Fluorescein-conjugated Antibody

Name: Human Common gamma Chain/IL-2 R gamma Fluorescein-conjugated Antibody
Brand: R&D Systems

Datasheet / CoA / SDS

Flow Cytometry

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Species Reactivity

Human
Specificity

Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with rhIL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross‑reactivity with rhIL-2 R alpha is observed.
Source

Monoclonal Mouse IgG_2B Clone # 633162
Purification

Protein A or G purified from hybridoma culture supernatant
Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Common gamma Chain/IL-2 R gamma
Leu23-Asn254
Accession # P31785
Formulation

Supplied in a saline solution containing BSA and Sodium Azide.
Label

Fluorescein

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of Common gamma Chain/IL‑2 R gamma in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human Common gamma Chain/IL‑2 R gamma Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2842F) or (B) Mouse IgG_2B Fluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 Receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid (aa) protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 Receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the Common gamma Chain ( gamma _c). The site of molecular defects in X-linked SCID (Severe Combined Immunodeficiency) has been mapped to the IL-2 R gamma gene.

References:
1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
2. Noguchi, M. et al. (1993) Science 262:1877.

Long Name:

IL-2 Receptor gamma

Entrez Gene IDs:

3561 (Human); 16186 (Mouse)

Alternate Names:

CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

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FAQs

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Isotype Controls

Description Application Cat# Citations Images

Mouse IgG2B Fluorescein‑conjugated Control Antibody

Ctrl IC0041F 7

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Staining Reagents

Description Application Cat# Citations Images

Flow Cytometry Staining Buffer (1X)

Flow FC001 7

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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5

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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1

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