Human Common  gamma  Chain/IL-2 R gamma Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB2842F
Detection of Common  gamma  Chain/IL‑2 R gamma  in Human Blood Lymphocytes by Flow Cytometry.
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Human Common  gamma  Chain/IL-2 R gamma Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Common gamma Chain/IL-2 R gamma in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R alpha, rhIL-2 R beta, rhIL-15 R alpha, recombinant mouse IL-2 R gamma, or recombinant canine IL-2 R alpha is observed. In Western blots, approximately 80% cross-reactivity with rhIL-2 R beta, 50% cross-reactivity with rhIL-15 R alpha and recombinant mouse IL-2 R alpha, and no cross‑reactivity with rhIL-2 R alpha is observed.
Source
Monoclonal Mouse IgG2B Clone # 633162
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf21-derived recombinant human Common gamma Chain/IL-2 R gamma
Leu23-Asn254
Accession # P31785
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Common  gamma  Chain/IL‑2 R gamma  in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of Common  gamma  Chain/IL‑2 R gamma in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human Common  gamma  Chain/IL‑2 R gamma Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2842F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional human IL-2 Receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid (aa) protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 Receptor signaling. Besides IL-2, the gamma  chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the Common gamma Chain ( gamma c). The site of molecular defects in X-linked SCID (Severe Combined Immunodeficiency) has been mapped to the IL-2 R gamma gene.

References
  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Entrez Gene IDs
3561 (Human); 16186 (Mouse)
Alternate Names
CD132 antigen; CD132; CIDX; combined immunodeficiency, X-linked; common cytokine receptor gamma chain; Common gamma Chain; common gamma-chain; cytokine receptor common subunit gamma; gamma(c); IL-2 R gamma; IL-2 receptor subunit gamma; IL2R gamma; IL-2R subunit gamma; IL2RG; IL-2RG; IMD4; interleukin 2 receptor, gamma; Interleukin-2 receptor subunit gamma; P64; SCIDX; SCIDX1; severe combined immunodeficiency

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