|Detection of Common gamma Chain/IL‑2 R gamma in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human Common gamma Chain/IL‑2 R gamma Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2842F) or (B) Mouse IgG2B Fluorescein Isotype Control (Catalog # IC0041F). View our protocol for Staining Membrane-associated Proteins.|
The gamma chain of the high affinity functional human IL-2 Receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid (aa) protein consisting of a 22 aa signal sequence, a 232 aa extracellular domain, a 29 aa transmembrane domain and an 86 aa cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL‑2 with any appreciable affinity, it is required for IL-2 Receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma has been designated the Common gamma Chain ( gamma c). The site of molecular defects in X-linked SCID (Severe Combined Immunodeficiency) has been mapped to the IL-2 R gamma gene.
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