|Detection of CRHR1 in BaF3 Mouse Cell Line Transfected with Human CRHR1 by Flow Cytometry. BaF3 mouse pro-B cell line transfected with human CRHR1 was stained with Mouse Anti-Human CRHR1 PE‑conjugated Monoclonal Antibody (Catalog # FAB3930P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). View our protocol for Staining Membrane-associated Proteins.|
Corticotrophin-Releasing Hormone Receptor 1 (CRHR1), also known as CRFR-1, is a 65-70 kDa, 7TM protein that belongs to the G protein-coupled receptor family 2. CRHR1 is expressed in multiple regions of the brain, where neurons in the cortex, amygdala, hippocampus and olfactory bulb all express CRHR1. Cells that express CRHR1 outside the CNS include adrenal fasciculata cells, neutrophils, adipocytes, pituitary corticotrophs, macrophages, testicular Leydig cells, and endometrial stromal cells. Functionally, the CRHR1 receptor mediates ACTH and beta -Endorphin release from the pituitary, VEGF secretion by mast cells, and BDNF expression by cerebellum neurons. The immunogen for this antibody corresponds to one of at least eight alternately spliced forms and shows a 29 amino acid deletion in the first cytoplasmic domain relative to the full-length isoform. Human CRHR1 shares 97% amino acid sequence identity with mouse and rat CRHR1.