Human CRTH-2 Fluorescein-conjugated Antibody

Discontinued Product

FAB3338F has been discontinued.
Detection  of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (1)
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Human CRTH-2 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CRTH‑2. Stains human CRTH-2-transfected cells but not parental cells.
Source
Monoclonal Mouse IgG2A Clone # 301108
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CRTH-2
Met1-Ser395
Accession # Q9Y5Y4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL‑3 R alpha /CD123 APC‑conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Mouse Anti-Human CRTH‑2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3338F) or (B) Mouse IgG2A Fluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CRTH-2

CRTH-2, also known as GPR44, is a 7TM G protein-coupled receptor that is expressed by activated Th2 cells and eosinophils. CRTH-2 binds Prostaglandin D2 and induces chemotaxis of these cells in allergic and inflammatory immune responses. Human and mouse CRTH-2 share 78% amino acid sequence identity.

Long Name
Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells
Entrez Gene IDs
11251 (Human); 14764 (Mouse); 309212 (Rat)
Alternate Names
CD294 antigen; CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2; CRTH-2; CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells; DL1R; DP2; G protein-coupled receptor 44; GPR44; PGD2R; PTGDR2; putative G-protein coupled receptor 44

Product Datasheets

Citation for Human CRTH-2 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.
    Authors: Chimen M, McGettrick H, Apta B, Kuravi S, Yates C, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock J, Cunningham A, Raza K, Filer A, Copland D, Dick A, Robinson J, Kalia N, Walker L, Buckley C, Nash G, Narendran P, Rainger G
    Nat Med, 0;21(5):467-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow

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