Human CRTH-2 Fluorescein-conjugated Antibody

FAB3338F has been discontinued. View all CRTH-2 products.
  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human CRTH‑2. Stains human CRTH-2-transfected cells but not parental cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 301108
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human CRTH-2
    Met1-Ser395
    Accession # Q9Y5Y4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CRTH‑2 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human IL‑3 R alpha /CD123 APC‑conjugated Monoclonal Antibody (Catalog # FAB301A) and either (A) Mouse Anti-Human CRTH‑2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3338F) or (B) Mouse IgG2A Fluorescein Isotype Control (Catalog # IC003F). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CRTH-2
CRTH-2, also known as GPR44, is a 7TM G protein-coupled receptor that is expressed by activated Th2 cells and eosinophils. CRTH-2 binds Prostaglandin D2 and induces chemotaxis of these cells in allergic and inflammatory immune responses. Human and mouse CRTH-2 share 78% amino acid sequence identity.
  • Long Name:
    Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells
  • Entrez Gene IDs:
    11251 (Human); 14764 (Mouse); 309212 (Rat)
  • Alternate Names:
    CD294 antigen; CD294; Chemoattractant receptor-homologous molecule expressed on Th2 cells; CRTH2; CRTH-2; CRTH2chemoattractant receptor homologous molecule expressed on T helper type 2 cells; DL1R; DP2; G protein-coupled receptor 44; GPR44; PGD2R; PTGDR2; putative G-protein coupled receptor 44
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Homeostatic regulation of T cell trafficking by a B cell-derived peptide is impaired in autoimmune and chronic inflammatory disease.
    Authors: Chimen M, McGettrick H, Apta B, Kuravi S, Yates C, Kennedy A, Odedra A, Alassiri M, Harrison M, Martin A, Barone F, Nayar S, Hitchcock J, Cunningham A, Raza K, Filer A, Copland D, Dick A, Robinson J, Kalia N, Walker L, Buckley C, Nash G, Narendran P, Rainger G
    Nat Med, 0;21(5):467-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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