Detects human CXCL8/IL‑8 in ELISAs and Western blots. In Western blots, this antibody shows 100% cross-reactivity with recombinant porcine CXCL8/IL‑8 and no cross-reactivity with recombinant rat CXCL3/CINC-2 beta.
Monoclonal Mouse IgG1 Clone # 6217
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CXCL8/IL‑8 Ser28-Ser99 Accession # P10145
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL‑8 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC208F) and Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P), as shown in panel A.Inhibition of IC208F staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002F). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
CXCL8/IL-8 is an 8-9 kDa member of the CXCL subfamily of chemokines. It was originally discovered and purified
independently by a number of laboratories as a neutrophil chemotactic and
activating factor. It has also referred to as Neutrophil Activating Protein
(NAP), Monocyte-derived Neutrophil Chemotactic Factor (MDNCF) and granulocyte
chemotactic protein (GCP). Many cell types, including monocyte/macrophages, T
cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes,
chondrocytes, and mammary plus alveolar epithelium can produce CXCL8 in
response to a wide variety of stimuli, such as IL-1b, TNF-a, LPS, MRP-8/14 and viruses. When secreted, it circulates as
both a monomer and dimer, with the dimeric forms being a homodimer and
heterodimer with CXCL4/PF4. CXCL8 binds
to CXCR-1 and -2, with the CXCL8 monomer favoring CXCR-1, and the CXCL8
homodimer favoring CXCR-2 and glycosaminoglycans. CXCL8 is a potent
chemoattractant for neutrophils. In addition, CXCL8 also has a wide range of
other pro-inflammatory effects. CXCL8 causes degranulation of neutrophil
specific granules and azurophilic granules. It also induces expression of the
cell adhesion molecules CD11/CD18, and enhances the adherence of neutrophils to
endothelial cells and sub-endothelial matrix proteins. Besides neutrophils,
CXCL8 is also chemotactic for basophils, T cells and eosinophils. CXCL8 has
been reported to be a co-mitogen for keratinocytes, and has also shown to be an
autocrine growth factor for melanoma cells. CXCL8 is also reported to be
angiogenic both in vivo and in vitro. There is no direct
structural rodent counterpart to human IL-8.
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