Intracellular Staining by Flow Cytometry
|Detection of CXCL8/IL‑8 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes treated with LPS were stained with Mouse Anti-Human CXCL8/IL‑8 PE‑conjugated Monoclonal Antibody (Catalog # IC208P) and Anti-Human CD14 Fluorescein-conjugated Antibody (panel A). Inhibition of IC208P staining by the addition of excess Recombinant Human IL-8 (Catalog # 208-IL) is shown in panel B. Quadrant markers were set based on control antibody staining (Catalog # IC002G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
CXCL8, also known as IL-8, is a CXC chemokine that is a potent chemoattractant for neutrophils and has a wide range of other pro-inflammatory effects.
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