Human DEC-205/CD205 Fluorescein-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human DEC‑205/CD205 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse DEC-205 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 523203
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human DEC‑205/CD205
    Cys216-Cys501
    Accession # O60449
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of DEC‑205/CD205 in Human Monocytes by Flow Cytometry. Human monocytes were stained with Mouse Anti-Human DEC‑205/CD205 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB2047F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: DEC-205/CD205
DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti-human DEC-205 antibody contains the first two C-type lectin domains.
  • Entrez Gene IDs:
    4065 (Human)
  • Alternate Names:
    CD205; DEC205; DEC-205; Ly75
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Fluorescein-conjugated Antibody

Ctrl IC002F 14  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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