Human Dectin-1/CLEC7A Fluorescein-conjugated Antibody

FAB1859F has been discontinued and is replaced by FAB1859G.
Clone 259931 was used by HLDA to establish CD designation
  (1 citations)     
Datasheet / CoA / SDS
Product Details
Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Detects human Dectin‑1/CLEC7A in direct ELISAs. In directs ELISAs, less than 10% cross-reactivity with recombinant human (rh) DLEC and no cross-reactivity with recombinant mouse Dectin-1 or rhDectin-2 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 259931
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Dectin‑1/CLEC7A
    Thr66-Met201
    Accession # NP_072092
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Fluorescein
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Dectin‑1/CLEC7A in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Dectin‑1/CLEC7A Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB1859F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 33 kDa type II transmembrane C-type lectin that participates in the innate immune response to fungal pathogens. Although Dectin-1 structurally resembles other CLEC molecules, it binds its ligands in a calcium-independent manner (1, 2). Mature human Dectin-1 consists of a short N-terminal ITAM-containing cytoplasmic tail, a transmembrane segment, and a C-terminal stalk with a carbohydrate recognition domain (CRD) in the extracellular domain (3, 4). Alternate splicing generates one major splice form that lacks the stalk region (3‑5). This isoform is expressed on the surface of monocytes, macrophages, myeloid DC, neutrophils, eosinophils, B cells, and CD4+ T cells (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (5‑7). Yeast beta -glucan is accessible to Dectin‑1 only during the process of cell budding. Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). In the membrane, Dectin-1 colocalizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 also interacts with tetraspanin CD37. This increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Dectin-1 knockout mice show increased susceptibility to pathogenic infection (15‑16). The CRD of human Dectin-1 shares 77%, 60%, and 60% amino acid (aa) sequence identity with that of bovine, mouse and rat Dectin-1, respectively. It shares 29%‑39% aa sequence identity with the CRD of other subgroup members, including CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL.

  • References:
    1. Kanazawa, N. (2007) J. Dermatol. Sci. 45:77.
    2. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33.
    3. Hernanz-Falcon, P. et al. (2001) Immunogenetics 53:288.
    4. Yokota, K. et al. (2001) Gene 272:51.
    5. Willment, J.A. et al. (2001) J. Biol. Chem. 276:43818.
    6. Willment, J.A. et al. (2005) Eur. J. Immunol. 35:1539.
    7. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771.
    8. Gantner, B.N. et al. (2005) EMBO J. 24:1277.
    9. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107.
    10. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467.
    11. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119.
    12. Yadav, M. and J.S. Schorey (2006) Blood 108:3168.
    13. Suram, S. et al. (2006) J. Biol. Chem. 281:5506.
    14. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154.
    15. Saijo, S. et al. (2007) Nat. Immunol. 8:39.
    16. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
  • Entrez Gene IDs:
    64581 (Human); 56644 (Mouse)
  • Alternate Names:
    Beta-glucan receptor; BGR; CD369; CLEC7A; CLECSF12; CLECSF12DC-associated C-type lectin 1; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12; C-type lectin domain family 7 member A; C-type lectin domain family 7, member A; C-type lectin superfamily member 12; Dectin1; Dectin-1; DECTIN1CANDF4; Dendritic cell-associated C-type lectin 1; dendritic cell-associated C-type lectin-1; hDectin-1; lectin-like receptor 1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. An unrestrained proinflammatory M1 macrophage population induced by iron impairs wound healing in humans and mice.
    Authors: Sindrilaru A, Peters T, Wieschalka S, Baican C, Baican A, Peter H, Hainzl A, Schatz S, Qi Y, Schlecht A, Weiss JM, Wlaschek M, Sunderkotter C, Scharffetter-Kochanek K
    J. Clin. Invest., 2011;121(3):985-97.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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