Human Dopamine D5 R/DRD5 PE-conjugated Antibody

FAB82861P has been discontinued. View all Dopamine D5 R/DRD5 products.
(1 Review)
  
  • Species Reactivity
    Human
  • Specificity
    Detects HEK293 human embryonic kidney cell line transfected with human Dopamine D5 R/DRD5 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.
  • Source
    Monoclonal Mouse IgG2A Clone # 889022
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human Dopamine D5 R/DRD5
    Met1-His477
    Accession # P21918
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Dopamine D5 R/DRD5 in HEK293 Human Cell Line Transfected with Human Dopamine D5 R/DRD5 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Dopamine D5 R/DRD5 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Dopamine D5 R/DRD5 PE‑conjugated Monoclonal Antibody (Catalog # FAB82861P). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Dopamine D5 R/DRD5
DRD5, also known as Dopamine D5 Receptor has a higher affinity for neurotransmitter dopamine than DRD1 (1). DRD5 is expressed in neurons in many human brain regions, including cortex regions, hippocampus, choroid plexus, and brainstem (2). Polymorphisms in the DRD5 gene have been associated with Attention Deficit Hyperactivity Disorder (ADHD) (3), schizopherenia (4) and nicotine dependence (5). Dopamine receptors undergo endocytosis upon interaction with receptor agonists and by activation of protein kinase C (PKC) (6).
  • References:
    1. Sunahra, R. et al. (1991) Nature. 350:614.
    2. Weinshank, R. et al. (1991) J Biol Chem. 266:22427.
    3. Squassina, A. et al.  (2008) Neurosci Lett. Feb 13.
    4. Golimbet, V. et al.  (2008) Bull Exp Biol Med. Jan.
    5. Wei, J. et al.  (2012) Addict Behav. 37:622.
    6. Thompson, D. et al. (2011) Traffic. 12:644.
  • Long Name:
    Dopamine D5 Receptor
  • Entrez Gene IDs:
    1816 (Human); 13492 (Mouse); 25195 (Rat)
  • Alternate Names:
    D(1B) dopamine receptor; D(5) dopamine receptor; D1beta dopamine receptor; DBDR; Dopamine D5 R; Dopamine D5 receptor; Dopamine D5R; dopamine receptor D1B; dopamine receptor D5; DRD1B; DRD1BDBDR; DRD1L2; DRD1L2MGC10601; DRD5
Related Research Areas
Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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Flow Cytometry Human Dopamine D5 R/DRD5 PE-conjugated Antibody FAB82861P
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 Flow Human Anonymous 06/20/2017
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Flow Cytometry Human Dopamine D5 R/DRD5 PE-conjugated Antibody FAB82861P
Flow Cytometry: Human Dopamine D5 R/DRD5 PE-conjugated Antibody [FAB82861P]

Summary

ApplicationFlow Cytometry
Sample TestedMV4-11,EOL-1 human acute myeloid (eosinophilic) leukemia cell line,KG-1 human acute myelogenous leukemia cell line,IMR-32 human neuroblastoma cell line
SpeciesHuman

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