Human E-Cadherin PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human E-Cadherin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 180224
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human E-Cadherin
    Accession # P12830
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of E-Cadherin in MCF-7 Human Cell Line by Flow Cytometry.

MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human E‑Cadherin PerCP‑conjugated Monoclonal Antibody (Catalog # FAB18381C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). Cells were stained in a buffer containing Ca2+ and Mg2+. View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: E-Cadherin

Epithelial (E)‑Cadherin (ECAD), also known as Cadherin-1, cell-CAM120/80 in the human, Uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules (gene name CDH1). Cadherins are calcium-dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E-Cadherin may also play a role in tumor development, as loss of E-Cadherin has been associated with tumor invasiveness. E-Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium‑dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E‑Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each (amino acids 155-697).

  • References:
    1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.
    2. Overduin, M. et al. (1995) Science 267:386.
    3. Takeichi, M. (1991) Science 251:1451.
  • Entrez Gene IDs:
    999 (Human); 12550 (Mouse); 83502 (Rat)
  • Alternate Names:
    Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PerCP-conjugated Isotype Control

Ctrl IC0041C
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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