Human EGF R/ErbB1 Fluorescein-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human EGF R/ErbB1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) ErbB2, rhErbB3, or rhErbB4 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 423103
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human EGF R/ErbB1
    Accession # CAA25240
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EGF R/ErbB1 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Rat Anti-Human EGF R/ErbB1 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB10951F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EGF R/ErbB1

Epidermal Growth Factor Receptor (EGF R), also named erythroblastic leukemia viral oncogene homolog 1 (ErbB1), is a member of the type I receptor tyrosine kinase superfamily. The epidermal growth factor receptor (EGF R) subfamily of receptor tyrosine kinases comprises four members: EGF R (also known as HER1, ErbB1or ErbB), ErbB2 (Neu, HER2), ErbB3 (HER3), and ErbB4 (HER4). All family members are type I transmembrane glycoproteins that have an extracellular domain with two ligand binding cysteine rich domains, separated by a spacer region, and a cytoplasmic domain with a membrane proximal tyrosine kinase domain and a C-terminal tail with multiple tyrosine autophosphorylation sites. The human EGF R gene encodes a 1210 amino acid (aa) residue precursor with a 24 aa putative signal peptide, a 621 aa extracellular domain, a 23 aa transmembrane domain, and a 542 aa cytoplasmic domain. EGF R has been shown to bind a subset of the EGF family ligands, including EGF, amphiregulin, TGF alpha, betacellulin, epiregulin, heparin-binding EGF and neuregulin-2 alpha, in the absence of a coreceptor. Ligand binding induces EGF R homodimerization as well as heterodimerization with ErbB2, resulting in kinase activation, tyrosine phosphorylation and cell signaling. EGF R can also be recruited to form heterodimers with ligand-activated ErbB3 or ErbB4. EGF R signaling has been shown to regulate multiple biological functions including cell proliferation, differentiation, motility and apoptosis. In addition, EGF R signaling has also been shown to play a role in carcinogenesis (1‑3).

  • References:
    1. Daly, R.J. (1999) Growth Factors, 16:255.
    2. Schlessinger, J. (2000) Cell. 103:211.
    3. Maihle, N.J. et al. (2002) Cancer Treat. Res. 107:247.
  • Long Name:
    Epidermal Growth Factor Receptor/Receptor Tyrosine Protein Kinase ErbB1
  • Entrez Gene IDs:
    1956 (Human); 13649 (Mouse)
  • Alternate Names:
    avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Efficacy of a non-hypercalcemic vitamin-D2 derived anti-cancer agent (MT19c) and inhibition of fatty acid synthesis in an ovarian cancer xenograft model.
    Authors: Moore RG, Lange TS, Robinson K
    PLoS ONE, 2012;7(4):e34443.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Fluorescein-conjugated Antibody

Ctrl IC006F 2  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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