Human EMR2 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human EMR2. Stains human EMR2-transfected cells but not the parental cell line.
  • Source
    Monoclonal Mouse IgG2B Clone # 494025
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with human EMR2
    Accession # AAI27006
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of EMR2 in Whole Blood Monocytes by Flow Cytometry. Whole blood monocytes were stained with Mouse Anti-Human EMR2 APC‑conjugated Monoclonal Antibody (Catalog # FAB4894A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: EMR2

EMR2 (EGF-like Module-containing Mucin-like Hormone Receptor 2), designated CD312, is a 90-100 kDa glycoprotein belonging to the EGF-TM7 family of adhesion-type class B 7‑transmembrane (TM) receptors. EGF-like sequences within long extracellular N-termini, and a GPS (G-protein proteolytic site) domain are characteristic of this family, which is mainly expressed on cells of the immune system (1-3). The human EMR2 cDNA encodes an 823 amino acid (aa) protein with five EGF-like domains within the first 250 aa, followed by a mucin-like stalk, a GPS domain (aa 479‑530) and a 7‑TM sequence (aa 531‑785). The GPS domain is the site of autocatalytic cleavage, forming a 70 kDa N-terminal, and a 30 kDa C-terminal portion that remains non-covalently attached as a heterodimer (2, 4). Of the first 290 aa of human EMR2, 284 aa (97%) are identical with family member CD97, likely due to gene duplication (3). The portion of human EMR2 N-terminal to the GPS domain (aa 1‑478) shares 64%, 59%, 48% and 45% aa identity with corresponding regions of canine EMR2, equine EMR2, mouse CD97 and rat CD97, respectively. There is no mouse ortholog for human EMR2. Alternate splicing of EMR2 creates isoforms that contain 2‑5 EGF-like domains. Only the 5-EGF form contains EGF4, which is necessary for calcium-dependent binding of the EMR2/CD97 ligand, chondroitin sulfate (CS) (3, 5‑7). None of the isoforms engage the CD97 ligand, CD55 (DAF). Notably, alternative trans-splicing produces a EMR2:CD97 hybrid where the third EGF-likek motif of CD97 is substituted for the third EGF-like motif of EMR2 (8). EMR2 is restricted to myeloid cells (2, 3). EMR2 expression increases as monocytes differentiate into macrophages, and decreases with differentiation into dendritic cells (6). Activation increases neutrophil EMR2 expression (6). EMR2 localizes to the leading edge of migrating neutrophils and plays an important role in migration, adhesion and superoxide production (9). It is also thought to facilitate specific interaction of myeloid cells with peripheral B lymphocytes which express CS (7).

  • References:
    1. Hamann, J. et al. (2015) Pharmocol. Rev. 67:338.
    2. Kwakkenbos, M.J. et al. (2004) Immunogenetics 55:655.
    3. Lin, H.-H. et al. (2000) Genomics 67:188.
    4. Lin, H.-H. et al. (2004) J. Biol. Chem. 279:31823.
    5. Stacey, M. et al. (2003) Blood 102:2916.
    6. Chang G.-W. et al. (2007) Biochem. Biophys. Res. Commun. 353:133.
    7. Kwakkenbos, M.J. et al. (2005) J. Leukoc. Biol. 77:112.
    8. Chiu, P-L. et al. (2008) FEBS Lett. 582:792.
    9. Yona, S. et al. (2008) FASEB J. 22:741.
  • Long Name:
    EGF-like Module Containing, Mucin-like, Hormone Receptor-like 2
  • Entrez Gene IDs:
    30817 (Human)
  • Alternate Names:
    CD312; DKFZp781B135; egf-like module containing, mucin-like, hormone receptor-like 2; egf-like module containing, mucin-like, hormone receptor-like sequence 2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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