|Detection of EphA2 in A431 Human Cell Line by Flow Cytometry. A431 human epithelial carcinoma cell line was stained with Mouse Anti-Human EphA2 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB3035C, filled histogram) or isotype control antibody (Catalog # IC003C, open histogram). View our protocol for Staining Membrane-associated Proteins.|
EphA2 (Erythropoietin-producing Hepatocellular Carcinoma-A2), also known as Eck, Myk2, and Sek2, is a 120-130 kDa member of the Ephrin receptor subfamily, tyrosine protein kinase family of molecules. It is one of at least 13 mammalian subfamily members that are divided into two groups (A and B) that are named for the corresponding class of ligand (Ephrins [Eph-receptor Interacting Protein]) to which they bind (1, 2). Mature EphA2 is a 953 amino acid (aa) type I transmembrane glycoprotein that contains a 514 aa extracellular domain (ECD). The ECD is characterized by the presence of one Eph ligand-binding domain and two fibronectin type III repeats. EphA2 binds Ephrins A1-A5, with subsequent receptor oligomerization and signal transduction (1, 2). It also exists, however, in a ligand-independent, auto-activated monomeric and inactive, homodimeric state, leading to complexities in its actions (3). Further, it is also known to form heterodimers with EphB6, a kinase-negative receptor that dampens EphA2 activity (4). EphA2 is expressed by a wide variety of cell types, including osteoblasts, neurons, capillary endothelium, Schwann cells, keratinocytes, Langerhans cells (LC) and IDC (mature LCs), cortical lens fiber cells and placenta-derived mesenchymal stem cells where EphA2 may serve as a biomarker (2, 5-11). Over aa 25-534, human and mouse EphA2 share 91% sequence identity.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.
The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided