Human ER beta /NR3A2 PE-conjugated Antibody

IC7106P has been discontinued. View all ER beta/NR3A2 products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human ER beta /NR3A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human ER alpha is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 733930
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ER beta /NR3A2
    Met1-Gly318
    Accession # Q92731
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of ER beta /NR3A2 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human ER beta /NR3A2 PE‑conjugated Monoclonal Antibody (Catalog # IC7106P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: ER beta/NR3A2
Estrogen Receptor beta (ER beta ; also known as NR3A2) is a member of the steroid receptor family. The natural ligand for ER is the classical estrogenic compound 17 beta ‑estradiol. ER beta is expressed in the granulosa cell layer of primary, secondary and mature follicles in the ovary, in bone, bladder, uterus, testis, epididymis, gastrointestinal tract, kidney, breast, heart, vessel wall, immune system, lung, pituitary, hippocampus and hypothalamus. Roles for ER beta in the reproductive and cardiovascular systems have been reported, although these are the subject of conflicting reports. ER beta has been postulated to act primarily as a modulator of ER alpha  function. ER beta has been shown to form homodimers as well as heterodimers with ER alpha. Both ER alpha and ER beta can give rise to numerous isoforms.
  • Long Name:
    Estrogen Receptor beta
  • Entrez Gene IDs:
    2100 (Human)
  • Alternate Names:
    ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2
Related Research Areas

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