Human FABP2/I-FABP Quantikine ELISA Kit

(3 Reviews)
  
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL)
  • Sensitivity
    6.21 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human FABP2
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC213, Quantikine Immunoassay Control Set 884 Human FABP2/I-FABP - Please Inquire
Product Summary
The Quantikine Human FABP2/I-FABP Immunoassay is a 4.5 hour solid phase ELISA designed to measure FABP2 levels in cell culture supernates, cell lysates, serum, plasma, and urine. It contains E. coli-expressed recombinant human FABP2 and antibodies raised against the recombinant protein. Results obtained for naturally occurring human FABP2 showed linear curves that were parallel to the standard curves obtained using the Quantikine Human FABP2 Immunoassay standards. These results indicate that this kit can be used to determine relative mass values for natural human FABP2.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020202020
Mean77.520742580.5216446
Standard Deviation3.147.2412.48.9417.426.6
CV%4.13.52.911.18.16

Recovery

The recovery of human FABP2 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 96-104
Cell Lysis Buffer (n=2) 83 81-85
EDTA Plasma (n=4) 97 85-102
Heparin Plasma (n=4) 95 96-104
Serum (n=4) 101 97-106
Urine (n=4) 90 85-95
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human FABP2/I-FABP Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: FABP2/I-FABP
Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.

FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes. Human FABP2 shares 78%, 82%, and 86% amino acid sequence identity with mouse, rat, and canine FABP2, respectively.

    • Long Name
      Fatty Acid-Binding Protein 2/Intestinal FABP
    • Entrez Gene IDs
      2169 (Human); 25598 (Rat);
    • Alternate Names
      FABP2; FABPI; fatty acid binding protein 2, intestinal; Fatty acid-binding protein 2; fatty acid-binding protein, intestinal; IFABP; I-FABP; I-FABPMGC133132; Intestinal FABP; Intestinal-type fatty acid-binding protein;
    Related Research Areas
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 884 Human FABP2/I-FABP

    Ctrl QC213
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 6
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    Cell Lysis Buffer 1 (1 x 21 mL)

    ELISA 890713 1
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    Average Rating: 4.7 (Based on 3 reviews)

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      Very Good
      Anonymous 10/20/2017
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    Summary

    Sample Testedblood and respiratory
     Human FABP2/I-FABP Quantikine ELISA Kit DFBP20
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    Excellent
      Anonymous 09/07/2017
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     Human FABP2/I-FABP Quantikine ELISA Kit DFBP20
    : Human FABP2/I-FABP Quantikine ELISA Kit [DFBP20]

    Summary

    Sample TestedSerum

    Other Experimental Details

    Other Experimental DetailsThe Quantikine ELISA kit worked as expected with low % CV's, and reproducible results.
     Human FABP2/I-FABP Quantikine ELISA Kit DFBP20
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    Excellent
      Anonymous 02/08/2017
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     Human FABP2/I-FABP Quantikine ELISA Kit DFBP20
    : Human FABP2/I-FABP Quantikine ELISA Kit [DFBP20]

    Summary

    Sample Testedhuman plasma

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