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Human Fas Ligand/TNFSF6 Quantikine ELISA Kit

  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sensitivity
    8.05 pg/mL
  • Assay Range
    15.60 - 1,000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma,)
  • Specificity
    Natural and recombinant human Fas Ligand
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human Fas Ligand Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fas Ligand in cell culture supernates, cell lysates, serum, and plasma. It contains CHO cell-expressed recombinant human Fas Ligand and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Fas Ligand showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Fas Ligand.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Inter-Assay Precision (Precision between assays Three samples of known concentration were tested on one plate to assess intra-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean10326162498.3261625
Standard Deviation4.810.7348.322.940
CV%4.74.15.48.48.86.4

Recovery

The recovery of Fas Ligand spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 94-109
EDTA Plasma (n=4) 99 90-114
Heparin Plasma (n=4) 98 89-115
Serum (n=4) 101 92-111
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Fas Ligand were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Fas Ligand/TNFSF6 Quantikine ELISA Kit
Background: Fas Ligand/TNFSF6
Fas Ligand is a 40 kDa type II transmembrane protein belonging to the TNF superfamily. The specific receptor for Fas Ligand is Fas (CD95, Apo-1). Fas Ligand is predominantly expressed on activated T cells and natural killer cells, while Fas is expressed on various types of cells. The Fas/Fas Ligand system plays a crucial role in modulating immune responses by inducing cell specific apoptosis. Human Fas Ligand shares 81% sequence identity with mouse Fas Ligand and 78% sequence identity with rat Fas Ligand.
    • Entrez Gene IDs
      356 (Human); 14103 (Mouse); 25385 (Rat);
    • Alternate Names
      CD178; CD95L; FASLG; TNFSF6; apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178; CD178 antigen; CD95-L; Fas antigen ligand; Fas ligand; Fas ligand (TNF superfamily, member 6); FASLCD95 ligand; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 20
    Filter your results:

    Sample Type
    1. Impaired thymic export and apoptosis contribute to regulatory T-cell defects in patients with chronic heart failure.
      Authors: Tang TT, Zhu ZF, Wang J, Zhang WC, Tu X, Xiao H, Du XL, Xia JH, Dong NG, Su W, Xia N, Yan XX, Nie SF, Liu J, Zhou SF, Yao R, Xie JJ, Jevallee H, Wang X, Liao MY, Shi GP, Fu M, Liao YH, Cheng X
      PLoS ONE, 2011;6(9):e24272.
      Species: Human
      Sample Type: Plasma
    2. Somatic FAS mutations are common in patients with genetically undefined autoimmune lymphoproliferative syndrome.
      Authors: Dowdell KC, Niemela JE, Price S, Davis J, Hornung RL, Oliveira JB, Puck JM, Jaffe ES, Pittaluga S, Cohen JI, Fleisher TA, Rao VK
      Blood, 2010;115(25):5164-9.
      Species: Human
      Sample Type: Plasma
    3. A 60-s postconditioning protocol by percutaneous coronary intervention inhibits myocardial apoptosis in patients with acute myocardial infarction.
      Authors: Zhao WS, Xu L, Wang LF, Zhang L, Zhang ZY, Liu Y, Liu XL, Yang XC, Cui L, Zhang L
      Apoptosis, 2009;14(10):1204-11.
      Species: Human
      Sample Type: Plasma
    4. The influence of hepatic damage on serum soluble Fas ligand levels of patients with drug rashes.
      Authors: Tohyama M, Shirakata Y, Sayama K, Hashimoto K
      J. Allergy Clin. Immunol., 2009;123(4):971-2; author.
      Species: Human
      Sample Type: Serum
    5. Differential effects of arsenic on cutaneous and systemic immunity: focusing on CD4+ cell apoptosis in patients with arsenic-induced Bowen's disease.
      Authors: Liao WT, Yu CL, Lan CC, Lee CH, Chang CH, Chang LW, You HL, Yu HS
      Carcinogenesis, 2009;30(6):1064-72.
    6. Granulysin is a key mediator for disseminated keratinocyte death in Stevens-Johnson syndrome and toxic epidermal necrolysis.
      Authors: Chung WH, Hung SI, Yang JY, Su SC, Huang SP, Wei CY, Chin SW, Chiou CC, Chu SC, Ho HC, Yang CH, Lu CF, Wu JY, Liao YD, Chen YT
      Nat. Med., 2008;14(12):1343-50.
      Species: Human
      Sample Type: blister fluid
    7. Vitamin C inhibits platelet expression of CD40 ligand.
      Authors: Pignatelli P, Sanguigni V, Paola SG, Lo Coco E, Lenti L, Violi F
      Free Radic. Biol. Med., 2005;38(12):1662-6.
      Species: Human
      Sample Type: Plasma
    8. Invasive trophoblasts stimulate vascular smooth muscle cell apoptosis by a fas ligand-dependent mechanism.
      Authors: Harris LK, Keogh RJ, Wareing M, Baker PN, Cartwright JE, Aplin JD, Whitley GS
      Am. J. Pathol., 2006;169(5):1863-74.
    9. Wheat gliadin induces apoptosis of intestinal cells via an autocrine mechanism involving Fas-Fas ligand pathway.
      Authors: D&amp;amp;apos;Archivio M
      FEBS Lett., 2003;540(1):117-24.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Normally occurring NKG2D+CD4+ T cells are immunosuppressive and inversely correlated with disease activity in juvenile-onset lupus.
      Authors: Dai Z, Turtle CJ, Booth GC, Riddell SR, Gooley TA, Stevens AM, Spies T, Groh V
      J. Exp. Med., 2009;206(4):793-805.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Erythropoiesis abnormalities contribute to early-onset anemia in patients with septic shock.
      Authors: Claessens YE, Fontenay M, Pene F, Chiche JD, Guesnu M, Hababou C, Casadevall N, Dhainaut JF, Mira JP, Cariou A
      Am. J. Respir. Crit. Care Med., 2006;174(1):51-7.
      Species: Human
      Sample Type: Plasma
    12. Growth hormone-induced reduction of soluble apoptosis mediators is associated with reverse cardiac remodelling and improvement of exercise capacity in patients with idiopathic dilated cardiomyopathy.
      Authors: Parissis JT, Adamopoulos S, Karatzas D, Paraskevaidis J, Livanis E, Kremastinos D
      Eur J Cardiovasc Prev Rehabil, 2005;12(2):164-8.
      Species: Human
      Sample Type: Plasma
    13. Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling.
      Authors: Beynon V, Quintana FJ, Weiner HL
      PLoS ONE, 2012;7(6):e39576.
    14. Escape from cardiomyocyte apoptosis by enterovirus persistence due to elevated soluble Fas-receptors.
      Authors: Alter P, Maisch B
      Z Kardiol, 2004;93(7):524-32.
      Species: Human
      Sample Type: Serum
    15. Functional polymorphisms in FAS and FASL contribute to increased apoptosis of tumor infiltration lymphocytes and risk of breast cancer.
      Authors: Zhang B, Sun T, Xue L, Han X, Zhang B, Lu N, Shi Y, Tan W, Zhou Y, Zhao D, Zhang X, Guo Y, Lin D
      Carcinogenesis, 2007;28(5):1067-73.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. High-dose glucose-insulin-potassium treatment reduces myocardial apoptosis in patients with acute myocardial infarction.
      Authors: Zhang L, Li YH, Zhang HY, Chen ML, Gao MM, Hu AH, Yang HS
      Eur. J. Clin. Invest., 2005;35(3):164-70.
      Species: Human
      Sample Type: Plasma
    17. Interleukin (IL)-4, IL-13, and IL-17A differentially affect the profibrotic and proinflammatory functions of fibrocytes from asthmatic patients.
      Authors: Bellini A, Marini MA, Bianchetti L, Barczyk M, Schmidt M, Mattoli S
      Mucosal Immunol, 0;5(2):140-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Effects of darbepoetin-alpha on plasma pro-inflammatory cytokines, anti-inflammatory cytokine interleukin-10 and soluble Fas/Fas ligand system in anemic patients with chronic heart failure.
      Authors: Kourea K, Parissis JT, Farmakis D, Panou F, Paraskevaidis I, Venetsanou K, Filippatos G, Kremastinos DT
      Atherosclerosis, 2007;199(1):215-21.
      Species: Human
      Sample Type: Plasma
    19. Isolation and characterization of CD146+ multipotent mesenchymal stromal cells.
      Authors: Sorrentino A, Ferracin M, Castelli G, Biffoni M, Tomaselli G, Baiocchi M, Fatica A, Negrini M, Peschle C, Valtieri M
      Exp. Hematol., 2008;36(8):1035-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Effect of quinapril on in-stent restenosis and relation to plasma apoptosis signaling molecules.
      Authors: Deftereos S, Giannopoulos G, Kossyvakis C, Kaoukis A, Raisakis K, Driva M, Panagopoulou V, Lappos S, Rentoukas I, Pyrgakis V, Alpert MA
      Am. J. Cardiol., 2010;105(1):54-8.
      Species: Human
      Sample Type: Plasma
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