Human Fc epsilon  RI alpha Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Fc epsilon RI alpha in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 773704
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Fc epsilon RI alpha
    Accession # P12319
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Fc epsilon  RI alpha in Human Peripheral Blood Cells by Flow Cytometry. Human peripheral blood cells were stained with Mouse Anti-Human ENPP‑3/CD203c APC‑conjugated Monoclonal Antibody (Catalog # FAB5756A) and either (A) Mouse Anti-Human Fc epsilon  RI alpha Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB6678G) or (B) Mouse IgG2B Alexa Fluor 488 Isotype Control (Catalog # IC0041G). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fc epsilon RI alpha

The alpha subunit of the high affinity IgE Receptor (Fc epsilon  RI alpha or Fc epsilon RIA) is an IgE‑binding type I transmembrane glycoprotein of the Multichain Immune Recognition (MIRR) family (1, 2). The receptor, Fc epsilon  RI, is a tetrameric complex of one alpha, one beta and two gamma subunits ( alpha beta gamma 2) on mast cells and basophils (1). An alternate trimeric form ( alpha gamma 2) is expressed on human, but not rodent, mast cells, basophils, eosinophils and professional antigen presenting cells (3). While the gamma subunit is essential for expression of Fc epsilon  RI alpha on the cell surface and for cell signaling, the beta subunit, when present, increases the halflife of the Fc epsilon  RI complex on the cell surface (3, 4). An isoform of the beta subunit, beta T, blocks processing of the alpha subunit and its cell surface expression (2, 3, 5). Human Fc epsilon  RI alpha cDNA encodes 257 amino acids (aa) including a 25 aa signal sequence, a 180 aa extracellular domain containing two Ig‑like domains that bind IgE and an endoplasmic reticulum retention motif, a 21 aa transmembrane domain with a charged amino acid (Asp219) that contributes to intracellular transport, and a 32 aa cytoplasmic sequence (1, 3, 6). Human Fc epsilon  RI alpha shares 50‑62% aa sequence identity with mouse, rat, equine, ovine, bovine, porcine and canine Fc epsilon  RI alpha. Binding of IgE alone increases surface expression of Fc epsilon  RI, while crosslinking of IgE/Fc epsilon  RI complexes by IgE ligands (allergens) initiates receptor internalization and signaling (2, 4, 5). Mast cell and basophil activation by IgE/Fc epsilon  RI crosslinking causes degranulation, releasing histamine, leukotrienes, prostaglandins, and other mediators of immediate‑type and late‑phase allergic reactions. Circulating autoantibodies that crosslink Fc epsilon  RI alpha are often found in patients with chronic urticaria (7). Fc epsilon  RI on human antigen presenting cells mediates uptake and processing of allergens for presentation by class II MHC (2, 3). Fc epsilon  RI expression on human DC and Langerhans cells is up‑regulated during allergic reactions (atopy) and correlates with serum IgE concentration (3).

  • References:
    1. Shimizu, A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1907.
    2. Abramson, J. and I. Pecht (2007) Immunol. Rev. 217:231.
    3. Kraft, S. and J-P. Kinet (2007) Nat. Rev. Immunol. 7:365.
    4. Yamasaki, S. and T. Saito (2008) J. Pharmacol. Sci. 106:336.
    5. Brenzovich, J. et al. (2009) J. Leukoc. Biol. 86:1351.
    6. Cauvi, D.M. et al. (2006) J. Biol. Chem. 281:10448.
    7. Kikuchi, Y. et al. (2001) J. Allergy Clin. Immunol. 107:1056.
  • Long Name:
    Fc epsilon Receptor I alpha
  • Entrez Gene IDs:
    2205 (Human); 14125 (Mouse); 25047 (Rat)
  • Alternate Names:
    FCE1A; FCER1A; FcERI; FceRIa
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC0041G  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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