Human Fc gamma  RI/CD64 PE-conjugated Antibody

FAB1257P has been discontinued. View all Fc gamma RI/CD64 products.
Clone 10.1 was used by HLDA to establish CD designation.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Fc gamma RI/CD64 in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # 10.1
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Rheumatoid synovial fluid cells and fibronectin purified monocytes
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Fc gamma  RI/CD64 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human Fc gamma  RI/CD64 PE‑conjugated Monoclonal Antibody (Catalog # FAB1257P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation. The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8  10‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10‑6  10‑7 M) only recognizing IgG aggregates surrounding multivalent antigens. Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domain, or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta. The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses. Three highly homologous genes (A, B, and C) sharing 98% identity at the nucleotide level have been identified for the human CD64 group. Fc gamma RI is a 68-70 kDa protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain. The genes for Fc gamma  RIB and Fc gamma  RIC contain stop codons within their membrane proximal Ig-like domains indicating possible secreted receptors. An mRNA splice variant of Fc gamma RIB has a deletion of the membrane-proximal Ig-like domain and encodes a putative transmembrane receptor. The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses. Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on mast cells, schwann cells, neutrophils and eosinophils. Its expression is up‑regulated during bacterial infections and sepsis. The extracellular domains of human and mouse Fc gamma  RI share 71% amino acid sequence identity.

  • Long Name:
    Fc gamma Receptor I
  • Entrez Gene IDs:
    2209 (Human); 2209 (Human); 14129 (Mouse); 295279 (Rat); 295279 (Rat); 102147198 (Cynomolgus Monkey); 102147198 (Cynomolgus Monkey)
  • Alternate Names:
    CD64 antigen; CD64; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FCRI; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I
Related Research Areas

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