Human Galanin PerCP-conjugated Antibody

IC5854C has been discontinued. View all Galanin products.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Galanin in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2B Clone # 581403
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E.coli-derived recombinant human Galanin
    Ala20-Ser123
    Accession # P22466
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Galanin in U‑87 MG Human Cell Line by Flow Cytometry. U‑87 MG human glioblastoma/astrocytoma cell line was stained with Mouse Anti-Human Galanin PerCP‑conjugated Monoclonal Antibody (Catalog # IC5854C, filled histogram) or isotype control antibody (Catalog # IC0041C, open histogram). Tofacilitate intracellular staining, cells were fixed with Flow CytometryFixation Buffer (Catalog # FC004)and permeabilized with Flow CytometryPermeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Galanin
Galanin (Glycine-Alanine, representing the N-and C-terminal amino acids in bovine Galanin; also GAL) is a 3 kDa (predicted), secreted member of the Galanin family of peptides. It is co-expressed with differing neuropeptides in a variety of neuron cell types. Galanin affects multiple metabolic processes by binding to one of three GPCRs. GalR1 blocks insulin secretion, GalR2 initiates neurogenesis and GalR3 influences addictive behavior. The human Galanin proform is 11‑12 kDa in size (predicted) and 104 amino acids (aa) in length. Proteolytic processing generates a 30 aa mature Galanin peptide (aa 33‑62), plus a phosphorylated (Ser117), C-terminal 59 aa GMAP fragment that is apparently involved in the processing of noxious stimuli. Once secreted, Galanin can undergo additional proteolytic degradation. Over aa 20‑123, the human Galanin proform shares 72% aa identity with mouse Galanin proform.
  • Entrez Gene IDs:
    51083 (Human); 14419 (Mouse); 29141 (Rat)
  • Alternate Names:
    GAL; GAL1; galanin prepropeptide; Galanin; GALN; GALNgalanin-related peptide; GLNNgalanin-message-associated peptide; GMAP; MGC40167
Related Research Areas

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