Human GATA-2 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human GATA-2. In direct ELISAs and Western blots, this antibody shows less than 1% cross-reactivity with rhGATA-1, rhGATA-5 and rhGATA-6.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human GATA-2
    Ala15-Thr279
    Accession # P23769
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed human umbilical vein endothelial cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Human GATA‑2 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and KG‑1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑2 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human GATA‑2 by Simple WesternTM. Simple Western lane view shows lysates of KG‑1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GATA‑2 at approximately 64 kDa (as indicated) using 5 µg/mL of Goat Anti-Human GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GATA-2
GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).
  • References:
    1. Tsai, S.F. et al. (1989) Nature 339:446.
    2. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.
  • Entrez Gene IDs:
    2624 (Human)
  • Alternate Names:
    endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA2; GATA-2; GATA-binding protein 2NFE1B; MGC2306
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development.
    Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R
    Dev Biol, 2016;409(1):218-33.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC-OCT embedded
  2. GATA2 germline mutations impair GATA2 transcription, causing haploinsufficiency: functional analysis of the p.Arg396Gln mutation.
    Authors: Cortes-Lavaud X, Landecho M, Maicas M, Urquiza L, Merino J, Moreno-Miralles I, Odero M
    J Immunol, 2015;194(5):2190-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ChIP
  3. The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice.
    Authors: Minami T, Yano K, Miura M, Kobayashi M, Suehiro J, Reid PC, Hamakubo T, Ryeom S, Aird WC, Kodama T
    J. Clin. Invest., 2009;119(8):2257-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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