Human Glut2 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1414G-100UG
Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry.
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Human Glut2 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes human Glut2 expression on human Glut2‑transfected NS0 cells, but not on control transfectants. Was shown to detect Glut2 on the surface of the human intestinal cell line Caco2 (2) in flow cytometry and on fixed cells in immunocytochemistry tests. Based on flow cytometric tests on transfected cells, this antibody has no cross-reactivity with human Glut1 or human Glut3.
Source
Monoclonal Mouse IgG2A Clone # 199017
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human Glut2
Met1-Val524
Accession # P11168
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Glut2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Glut2 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1414G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Glut2

Glut2 belongs to the facilitative glucose transporter protein family that comprises 13 members. It is an integral membrane protein with 12 transmembrane domains. Glut2 is expressed predominantly in liver, intestine, kidney, and pancreatic beta-cells. It is a low-affinity glucose transporter that has been suggested to function as a glucose sensor in pancreatic beta-cells and facilitate either glucose uptake or efflux from cells depending on the nutritional state (1).

References
  1. Olson, A.L. and J.E. Pessin (1996) Annu. Rev. Nut. 16:235.
  2. Mahraoui, L. et al. (1994) J. Biochem. 298:629.
Long Name
Glucose Transporter Type 2
Entrez Gene IDs
6514 (Human); 20526 (Mouse)
Alternate Names
GLUT2 Glucose transporter type 2, liver; Glut2; GLUT-2; SLC2A2; solute carrier family 2 (facilitated glucose transporter), member 2; solute carrier family 2, facilitated glucose transporter member 2

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