Human gp130 PerCP-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human gp130 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-2 R, rhIL-4 R, or rhIL-6 R is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 28126
  • Purification
    Protein A or G purified from ascites
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human gp130 extracellular domain
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of gp130 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human gp130 PerCP-conjugated Monoclonal Antibody (Catalog # FAB228C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: gp130
Glycoprotein 130 (gp130; also known as IL-6 signal transducer, IL-6 receptor beta, oncostatin-M alpha subunit) is a ubiquitously expressed, 130 kDa type I transmembrane glycoprotein and member of the type II subfamily, type I cytokine receptor family. Functionally, it is responsible for transduction of the IL-6 signal across the plasma membrane (1). Rat gp130 is synthesized as a 918 amino acid (aa) precursor with a 22 aa signal sequence, a 596 aa extracellular domain (ECD), a 22 aa transmembrane region, and a 278 aa cytoplasmic tail. Eleven potential N-linked glycosylation sites are found within the rat gp130 ECD (1). The ECD also contains an N terminal immunoglobulin (Ig)-like C2-type domain, followed by the cytokine receptor homology region (CHR) which is made up of two fibronectin type III-like domains and a WSXWS motif, and three additional fibronectin type III-like domains (2). The domains in the CHR are the structural hallmarks of the hematopoietic cytokine receptor family (2). Human gp130 shares 73% and 79% aa sequence identity with mouse and rat gp130, respectively. Gp130 serves as the signal transducing receptor subunit for the IL-6-type cytokines consisting of interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), ciliary neurotrophic factor (CNTF), new neurotrophin factor-1 (NNT-1), IL-27, cardiotrophin-1 (CT-1), and cardiotrophin like cytokine (CLC) (2-5). These cytokines are involved in a variety of functions including the modulation of inflammatory and immune responses, heart development, fertility, and many other activities (2).
  • References:
    1. Wang, Y. et al. (1992) Genomics 14:666.
    2.  Muller-Newen, G. (2003) Sci. STKE pe40.
    3.  Heinrich, P.C. et al. (2003) Biochem. J. 374:1.
    4.  Stuhlmann-Laeisz, C. et al. (2006) Mol. Biol. Cell 17:2986.
    5.  Fischer, P. and D. Hilfiker-Kleiner (2008) Br. J. Pharmacol. 153:S414.
  • Long Name:
    Glycoprotein 130
  • Entrez Gene IDs:
    3572 (Human); 16195 (Mouse); 25205 (Rat)
  • Alternate Names:
    CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; GP130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PerCP-conjugated Antibody

Ctrl IC002C  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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