Detection of Granzyme A in CD56+ Human Blood Cells by Flow Cytometry.
CD56+ human whole blood cells were stained with Mouse Anti-Human Granzyme A Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC29051F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Granzyme A
Granzyme A is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A is the most abundant protease in CTL and NK cells. It induces caspase‑independent cell death when introduced into target cells by perforin (1). Human Granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1‑26), a propeptide (residues 27‑28) and a mature chain (residues 29‑262) (2). The purified recombinant human Granzyme A consists of residues 26 to 262. After being activated by Lysyl Endopeptidase, it cleaves a thioester substrate.
Lieberman, J. and Z. Fan (2003) Curr. Opin. Immunol. 15:553.
Gershenfeld, H.K. et al. (1988) Proc. Natl. Acad. Sci. USA 85:1184.
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