Human HAI-1 Ectodomain Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human HAI-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse
    HAI‑1 is observed and less than 1% cross-reactivity with recombinant human HAI-2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human HAI-1
    Pro37-Glu449
    Accession # NP_003701
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human HAI‑1 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human HAI‑1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for HAI‑1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunohistochemistry
HAI‑1 in Human Lung Cancer Tissue. HAI‑1 was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Goat Anti-Human HAI‑1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human HAI‑1 by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for HAI‑1 at approximately 88 kDa (as indicated) using 10 µg/mL of Goat Anti-Human HAI‑1 Ectodomain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1048) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HAI-1

HAI-1 is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matriptase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

  • References:
    1. Denda, K. et al. (2002) J. Biol. Chem. 277:14053. 
    2. Shimomura, T. et al. (1997) J. Biol. Chem. 272:6370.
    3. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.
  • Long Name:
    HGF Activator Inhibitor Type 1
  • Entrez Gene IDs:
    6692 (Human); 20732 (Mouse)
  • Alternate Names:
    HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Phosphorylation of the type II transmembrane serine protease, TMPRSS13 in Hepatocyte Growth Factor Activator Inhibitor-1 and 2-mediated cell surface localization
    Authors: AS Murray, FA Varela, TE Hyland, AJ Schoenbeck, JM White, LM Tanabe, SV Todi, K List
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Prostasin is required for matriptase activation in intestinal epithelial cells to regulate closure of the paracellular pathway.
    Authors: Buzza M, Martin E, Driesbaugh K, Desilets A, Leduc R, Antalis T
    J Biol Chem, 2013;288(15):10328-37.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development.
    Authors: Szabo R, Molinolo A, List K, Bugge TH
    Oncogene, 2006;26(11):1546-56.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
Cell and Tissue Staining Kits
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Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

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Antigen Retrieval Reagent Sampler (50 mL each)

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Antigen Retrieval Reagent-Acidic

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VCTS022
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VCTS021
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VCTS023
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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