Human Hyaluronidase 1/HYAL1 DuoSet ELISA

R&D Systems | Catalog # DY7358

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

12.5-800 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Hyaluronidase 1/HYAL1 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Hyaluronidase 1/HYAL1 ELISA Kits »

Product Summary for Human Hyaluronidase 1/HYAL1 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Hyaluronidase 1 / HYAL1. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Hyaluronidase 1/HYAL1 DuoSet ELISA

Human Hyaluronidase 1 / HYAL1 ELISA Standard Curve

Human Hyaluronidase 1 / HYAL1 ELISA Standard Curve

Kit Contents for Human Hyaluronidase 1/HYAL1 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

DuoSet Ancillary Reagent Kit 2 (5 plates): (Catalog # DY008C) containing 96 well microplates, plate sealers, substrate solution, stop solution, plate coating buffer (PBS), wash buffer, and Reagent Diluent Concentrate 2.

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: ELISA TMB Substrate (Catalog # DY999B or DY999B-250)

Stop Solution: Methanesulfonic acid (Catalog # DY994B or DY994B-250)

Microplates: (Catalog # DY990), or equivalent

Plate Sealers: (Catalog # DY992), or equivalent

*For the recommended Reagent Diluent and Blocking Buffer for a specific DuoSet ELISA Development Kit, refer to the product datasheet.

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Hyaluronidase 1/HYAL1

HYAL1 is one of five hyaluronidases with endo-beta-N-acetyl-hexosaminidase activity. It is a lysosomal enzyme with optimal activity around pH 4.0. It is highly expressed in the liver, kidney and heart, and it is the predominant hyaluronidase found in plasma. Defects in HYAL1 are associated with the hyaluronidase deficiency, mucopolysaccharidosis type IX. HYAL1 exhibits both tumor promoter and suppressor activity. While HYAL1, HYAL2, and SPAM1 are endo- beta-N-acetyl-glucosaminidases that primarily act on hyaluronan, HYAL4 is primarily active on chondroitin sulfate (types C and D in particular).

Long Name

Hyaluronoglucosaminidase-1

Alternate Names

FUS2, HYAL1, Hyaluronoglucosaminidase 1, LUCA1, NAT6

Entrez Gene IDs

3373 (Human); 15586 (Mouse); 367166 (Rat)

Gene Symbol

HYAL1

Additional Hyaluronidase 1/HYAL1 Products

Product Documents for Human Hyaluronidase 1/HYAL1 DuoSet ELISA

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Hyaluronidase 1/HYAL1 DuoSet ELISA

For research use only

Citations for Human Hyaluronidase 1/HYAL1 DuoSet ELISA

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