Human IGF-II Quantikine ELISA Kit

  
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  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Serum-free Cell Culture Supernates (25 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (25 uL)
  • Sensitivity
    5.41 pg/mL
  • Assay Range
    12.5 - 800 pg/mL (Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Urine)
  • Specificity
    Natural and recombinant human IGF-II.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC230 , Quantikine Immunoassay Control Set 918 for Human IGF-II - Please Inquire
Product Summary
The Quantikine® Human IGF-II Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGF-II in cell culture supernates, serum, plasma, and urine. It contains E. coli-expressed recombinant human IGF-II and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGF-II showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGF-II.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Serum-free Cell Culture Supern, Serum, EDTA Plasma, Heparin Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 128 305 566 136 307 558
Standard Deviation 4.05 7.2 15.6 9.89 28.5 28.2
CV% 3.2 2.4 2.8 7.3 9.3 5.1

Recovery

The recovery of human IGF-II spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Serum-free Cell Culture Media (n=4) 95 82-104
Urine (n=4) 94 79-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human IGF-II were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Human IGF-II Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IGF-II

Insulin-like growth factor (IGF)-I (also known as somatomedin C and somatomedin A) and IGF-II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved between the human, bovine and porcine proteins (100% identity), and exhibit cross-species activity.

The IGFL (insulin-like growth factor-like) family includes four small (~11 kDa), probably secreted family members in humans and one in mouse. This family shares A and B chain cysteine motifs with the IGF superfamily, and has an additional cysteine motif within an uncleaved region corresponding to the C peptide of the IGF family.

  • Long Name:
    Insulin-like Growth Factor II
  • Entrez Gene IDs:
    3481 (Human); 16002 (Mouse)
  • Alternate Names:
    C11orf43; chromosome 11 open reading frame 43; FLJ22066; FLJ44734; IGF2; IGF-2; IGFII; IGF-II; insulin-like growth factor 2 (somatomedin A); insulin-like growth factor II; insulin-like growth factor type 2; MSA; PP9974; somatomedin-A
Related Research Areas

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ELISA Controls
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Quantikine Immunoassay Control Set 918 for Human IGF-II

QC230
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

ELISA WA126 6
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