Human IGF-II R Fluorescein-conjugated Antibody

(1 citations)   
  • Species Reactivity
  • Specificity
    Detects human IGF-II R in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IGF-II R
    Accession # P11717
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IGF‑II R in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Goat Anti-Human IGF‑II R Fluorescein‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB2447F, filled histogram) or isotype control antibody (Catalog # IC108F, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IGF-II R

The type 2 Insulin-like Growth Factor Receptor (IGF-II R; also known as cation-independent mannose-6 phosphate receptor/CI-MPR) is a 300 kDa member of the P-type lectin family of molecules. P-type lectins generate functional eukaryotic lysosomes by binding and sorting lysosomal enzymes expressing phosphorylated mannose residues (M6P) (1-3). IGF-II R is a type I transmembrane glycoprotein that contains a 2,264 amino acid (aa) extracellular region, a 23 aa transmembrane segment and a 124 aa cytoplasmic tail (4, 5). The extracellular region consists of 15 contiguous “binding” repeats of about 150 aa each. The odd-numbered repeats interact with “ligands” while the even-numbered repeats likely generate a nondisulfide homodimer in the membrane (1). Repeat #11 binds IGF‑II, while repeats 3 and 9 bind mannose-6 phosphate; repeat #13 contains a fibronectin type II motif and assists in IGF-II binding (1, 2). In the extracellular region of IGF‑II R expressed by R&D Systems (600 amino acids), human IGF-II R is 85% identical to both mouse and bovine IGF-II R. This expressed region includes binding repeats #11, 12, and 13. In addition to IGF-II, CI-MPR/IGF-II R binds non-M6P containing ligands such as retinoic acid, urokinase-type plasminogen-activator receptor and plasminogen, plus M6P-containing molecules such as lysosomal enzymes, TGF-beta 1 precursor, proliferin, LIF, CD26, herpes simplex glycoprotein D, and granzymes A and B (2, 6). IGF-II R regulates many diverse biological functions that range from intracellular trafficking to the internalization of extracellular factors and modulation of cellular responses. It delivers newly synthesized M6P-tagged lysosomal enzymes from the trans-golgi network to endosomes, and facilitates the clearance of extracellular lysosomal and matrix degrading enzymes by internalization into clathrin-coated vesicles and delivery into endosomes. With respect to IGF-II biology, it would appear that IGF-II R is principally a regulator of local IGF-II levels, targeting IGF-II for destruction in lysosomes (2). However, some evidence suggests the receptor will signal via G-proteins, an effect that has yet to be conclusively shown (6).

  • References:
    1. Ghosh, P. et al. (2003) Nat. Rev. Mol. Cell. Biol. 4:202.
    2. Dahms, N.M. and M.K. Hancock (2002) Biochim. Biophys. Acta. 1572:317.
    3. Zaina, S. and J. Nilsson (2003) Curr. Opin. Lipidol. 14:483.
    4. Morgan, D.O. et al. (1987) Nature 329:301.
    5. Oshima, A. et al. (1988) J. Biol. Chem. 263:2553.
    6. Hawkes, C. and S. Kar (2004) Brain Res. Rev. 44:117.
  • Long Name:
    Insulin-like Growth Factor II Receptor
  • Entrez Gene IDs:
    3482 (Human)
  • Alternate Names:
    300 kDa mannose 6-phosphate receptor; cation-independent mannose-6 phosphate receptor; cation-independent mannose-6-phosphate receptor; CD222 antigen; CD222; CI Man-6-P receptor; CIMPR; CI-MPR; IGF2R; IGF-II receptor; IGFIIR; IGF-IIR; insulin-like growth factor 2 receptorM6P/IGF2R; Insulin-like growth factor II receptor; M6P/IGF2 receptor; M6PR; M6P-R; MPR 300; MPR1; MPRI; MPRIM6PR
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. Co-targeting the IGF system and HIF-1 inhibits migration and invasion by (triple-negative) breast cancer cells.
    Authors: Mancini M, Gariboldi M, Taiana E, Bonzi M, Craparotta I, Pagin M, Monti E
    Br J Cancer, 2014;110(12):2865-73.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Fluorescein-conjugated Control

Ctrl IC108F 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human IGF-II R Fluorescein-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Associated Pathways


Customer Information