Human IGSF8/CD316 MAb (Clone 2587G) Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human FFAR3/GPR41 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human FFAR3/GPR41 Monoclonal Antibody (Catalog # MAB10562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for FFAR3/GPR41 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of FFAR3/GPR41 in U937 Human Cell Line by Flow Cytometry U937 human histiocytic lymphoma cell line was stained with Rabbit anti-human FFAR3/GPR41 monoclonal antibody (Catalog # MAB10562, open histogram) or isotype control antibody (MAB1050, open histogram) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-Associated Proteins protocol.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
GPR41 and GPR42 are two closely related genes that are part of a cluster of four adjacent G protein-coupled receptors(GPR40, 41, 42, and 43). There are only six nucleotide and amino acid differences between GPR41 and GPR42. GPR41 is a receptor for short chain fatty acids. The rank order of potency for agonists of this receptor ispropionate = pentanoate= butyrate > acetate > formate. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation.
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