Human IGSF8/CD316 MAb (Clone 2587G)

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB10562-100
MAB10562-SP
Detection of Human FFAR3/GPR41 by Western Blot.
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Human IGSF8/CD316 MAb (Clone 2587G) Summary

Species Reactivity
Human
Specificity
Detects human FFAR3 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2590G
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing human FFAR3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
A549 human lung carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Flow Cytometry
0.25 µg/106 cells
U937 human histiocytic lymphoma cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Human FFAR3/GPR41 by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human FFAR3/GPR41 Monoclonal Antibody (Catalog # MAB10562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for FFAR3/GPR41 at approximately 39 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Flow Cytometry Detection of FFAR3/GPR41 antibody in U937 Human Cell Line antibody by Flow Cytometry View Larger

Detection of FFAR3/GPR41 in U937 Human Cell Line by Flow Cytometry U937 human histiocytic lymphoma cell line was stained with Rabbit anti-human FFAR3/GPR41 monoclonal antibody (Catalog # MAB10562, open histogram) or isotype control antibody (MAB1050, open histogram) followed by Allophycocyanin-conjugated anti-Rabbit IgG Secondary Antibody (F0111). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FFAR3/GPR41

GPR41 and GPR42 are two closely related genes that are part of a cluster of four adjacent G protein-coupled receptors(GPR40, 41, 42, and 43). There are only six nucleotide and amino acid differences between GPR41 and GPR42. GPR41 is a receptor for short chain fatty acids. The rank order of potency for agonists of this receptor ispropionate = pentanoate= butyrate > acetate > formate. The activity of this receptor is coupled to the formation of inositol 1,4,5-trisphosphate, intracellular Ca2+ mobilization, the activation of ERK 1/2 and inhibition of intracellular cAMP accumulation.

Long Name
Free Fatty Acid Receptor 3
Entrez Gene IDs
2865 (Human); 233080 (Mouse); 365228 (Rat)
Alternate Names
FFA3; FFA3R; FFAR3; free fatty acid receptor 3; G protein-coupled receptor 41; GPR41; GPR41FFA3R; G-protein coupled receptor 41

Product Datasheets

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Human IGSF8/CD316 MAb (Clone 2587G)
By Anonymous on 09/21/2021
Application: WB Sample Tested: A549 human lung carcinoma cell line Species: Human