Detects human IL-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse IL-11 is observed and no cross-reactivity with recombinant human (rh) IL-6, rhCNTF, rhLIF, rhOSM, rhCLC, or rhCT-1 is observed.
Monoclonal Mouse IgG2A Clone # 22626
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IL‑11 Pro22-Leu199 Accession # P20809
Supplied in a saline solution containing BSA and Sodium Azide.
Intracellular Staining by Flow Cytometry
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑11 in MRC‑5 Human Cell Line by Flow Cytometry. MRC‑5 human embryonic lung fibroblast cell line treated with histamine and Recombinant Human TGF‑ beta 1 (Catalog # 240-B) was stained with Mouse Anti-Human IL‑11 PE‑conjugated Monoclonal Antibody (Catalog # IC218P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
(IL-11) is a pleiotropic cytokine produced by stromal cells. It binds to a dimeric
receptor complex consisting of the IL-11 Ra
chain and the gp130 chain shared by the receptors for the IL-6 cytokine family.
IL-11 has multiple effects on hematopoietic cells and osteoblasts.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.