Human IL-11 PE-conjugated Antibody

IC218P has been discontinued. View all IL-11 products.
  (2 citations)     
Datasheet / CoA / SDS
Product Details
Citations (2)
FAQs
Reviews
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant mouse IL-11 is observed and no cross-reactivity with recombinant human (rh) IL-6, rhCNTF, rhLIF, rhOSM, rhCLC, or rhCT-1 is observed.
  • Source
    Monoclonal Mouse IgG2A Clone # 22626
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IL‑11
    Pro22-Leu199
    Accession # P20809
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Intracellular Staining by Flow Cytometry
Detection of IL‑11 in MRC‑5 Human Cell Line by Flow Cytometry. MRC‑5 human embryonic lung fibroblast cell line treated with histamine and Recombinant Human TGF‑ beta 1 (Catalog # 240-B) was stained with Mouse Anti-Human IL‑11 PE‑conjugated Monoclonal Antibody (Catalog # IC218P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-11
Interleukin-11 (IL-11) is a pleiotropic cytokine produced by stromal cells. It binds to a dimeric receptor complex consisting of the IL-11 Ra chain and the gp130 chain shared by the receptors for the IL-6 cytokine family. IL-11 has multiple effects on hematopoietic cells and osteoblasts.
  • Long Name:
    Interleukin 11
  • Entrez Gene IDs:
    3589 (Human); 16156 (Mouse); 171040 (Rat)
  • Alternate Names:
    Adipogenesis inhibitory factor; AGIF; AGIFoprelvekin; IL11; IL-11; IL-11Oprelvekin; interleukin 11; interleukin-11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. IL-11 Induces Th17 Cell Responses in Patients with Early Relapsing-Remitting Multiple Sclerosis.
    Authors: Zhang X, Tao Y, Chopra M, Dujmovic-Basuroski I, Jin J, Tang Y, Drulovic J, Markovic-Plese S
    J Immunol, 2015;194(11):5139-49.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.
    Authors: Zdzisinska B, Bojarska-Junak A, Dmoszynska A, Kandefer-Szerszen M
    Arch. Immunol. Ther. Exp. (Warsz.), 2008;56(3):207-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

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