Human IL-15 QuantiGlo ELISA Kit

Name: Human IL-15 QuantiGlo ELISA Kit
Brand: R&D Systems
SKU: Q1500B

R&D Systems | Catalog # Q1500B

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Product Documents

Discontinued Product

Q1500B has been discontinued. View all IL-15 products.

Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

5.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)

Sensitivity

0.431 pg/mL

Assay Range

1.03-750 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)

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Product Summary for Human IL-15 QuantiGlo ELISA Kit

The QuantiGlo Human IL-15 Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-15 levels in cell culture supernates, serum, and plasma. It contains recombinant human IL-15 expressed in E. coli and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-15 showed linear curves that were parallel to the standard curves obtained using the recombinant QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-15.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Chemiluminescent - Luminometer (Luminol)

Species

Human

Specificity

Natural and recombinant human IL-15

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	5.9	60.3	420	4.59	55.4	419
Standard Deviation	0.22	1.80	11.7	0.60	5.82	27.4
CV%	3.7	3.0	2.8	13.1	10.5	6.5

EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	40	40	40
Mean (pg/mL)	4.89	73.9	499	4.93	61.3	456
Standard Deviation	0.26	2.90	18.0	0.65	7.69	41.1
CV%	5.3	3.9	3.6	13.2	12.5	9.0

Recovery for Human IL-15 QuantiGlo ELISA Kit

The recovery of IL-15 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	102	97-110
EDTA Plasma (n=4)	105	87-114
Heparin Plasma (n=4)	105	88-119
Serum (n=4)	105	90-119

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-15 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human IL-15 QuantiGlo ELISA Kit

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-15

IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.