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Human IL-15 QuantiGlo ELISA Kit

Catalog #: Q1500B
7 Citations Datasheet / COA / SDS

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Q1500B has been discontinued.
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Citations (7)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human IL-15 QuantiGlo ELISA Kit Summary

Assay Length
5.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sensitivity
0.431 pg/mL
Assay Range
1.0 - 750 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human IL-15
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The QuantiGlo Human IL-15 Chemiluminescent Immunoassay is a 5.5 hour solid phase ELISA designed to measure human IL-15 levels in cell culture supernates, serum, and plasma. It contains recombinant human IL-15 expressed in E. coli and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IL-15 showed linear curves that were parallel to the standard curves obtained using the recombinant QuantiGlo kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-15.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 5.9 60.3 420 4.59 55.4 419
Standard Deviation 0.22 1.8 11.7 0.6 5.82 27.4
CV% 3.7 3 2.8 13.1 10.5 6.5

Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 4.89 73.9 499 4.93 61.3 456
Standard Deviation 0.26 2.9 18 0.65 7.69 41.1
CV% 5.3 3.9 3.6 13.2 12.5 9

Recovery

The recovery of IL-15 spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 102 97-110
EDTA Plasma (n=4) 105 87-114
Heparin Plasma (n=4) 105 88-119
Serum (n=4) 105 90-119

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-15 in various matrices were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IL-15

IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation, exhibits anti-tumor activity, and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha.

Long Name:
Interleukin 15
Entrez Gene IDs:
3600 (Human); 16168 (Mouse); 25670 (Rat); 102119613 (Cynomolgus Monkey); 493682 (Feline)
Alternate Names:
IL15; IL-15; IL-15MGC9721; interleukin 15; interleukin-15

Citations for Human IL-15 QuantiGlo ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Intramuscular delivery of heterodimeric IL-15 DNA in macaques produces systemic levels of bioactive cytokine inducing proliferation of NK and T cells.
    Authors: Bergamaschi C, Kulkarni V, Rosati M, Alicea C, Jalah R, Chen S, Bear J, Sardesai N, Valentin A, Felber B, Pavlakis G
    Gene Ther, 2014-10-02;22(1):76-86.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  2. Natural killer (NK) cell deficit in coronary artery disease: no aberrations in phenotype but sustained reduction of NK cells is associated with low-grade inflammation.
    Authors: Backteman K, Ernerudh J, Jonasson L
    Clin Exp Immunol, 2014-01-01;175(1):104-12.
    Species: Human
    Sample Types: Plasma
  3. The Magnitude and Kinetics of the Mucosal HIV-Specific CD8+ T Lymphocyte Response and Virus RNA Load in Breast Milk.
    Authors: Mahlokozera T, Kang HH
    PLoS ONE, 2011-08-23;6(8):e23735.
    Species: Human
    Sample Types: Milk
  4. IL-15 delays suppression and fails to promote immune reconstitution in virally suppressed chronically SIV-infected macaques.
    Authors: Lugli E, Mueller YM, Lewis MG
    Blood, 2011-07-14;118(9):2520-9.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma
  5. Interleukin-15 contributes to the regulation of murine adipose tissue and human adipocytes.
    Authors: Barra NG, Reid S, MacKenzie R
    Obesity (Silver Spring), 2009-12-17;18(8):1601-7.
    Species: Human
    Sample Types: Serum
  6. Influences of genetic variants in interleukin-15 gene and serum interleukin-15 levels on coronary heart disease.
    Authors: Gokkusu C, Aydin M, Ozkok E, Tulubas F, Elitok A, Pamukcu B, Umman B
    Cytokine, 2009-09-30;49(1):58-63.
    Species: Human
    Sample Types: Plasma
  7. Increased immune responses in rhesus macaques by DNA vaccination combined with electroporation.
    Authors: Rosati M, Valentin A, Jalah R, Patel V, von Gegerfelt A, Bergamaschi C, Alicea C, Weiss D, Treece J, Pal R, Markham PD, Marques ET, August JT, Khan A, Draghia-Akli R, Felber BK, Pavlakis GN
    Vaccine, 2008-04-21;26(40):5223-9.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Plasma

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