Human IL-17C Fluorescein-conjugated Antibody

Discontinued Product

IC1234F has been discontinued.
Detection  of IL‑17C in PC‑3 Human Cell Line by Flow Cytometry.
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Human IL-17C Fluorescein-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-17C in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human IL-17A, B, D, E or F is observed.
Source
Monoclonal Mouse IgG2A Clone # 177114
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL‑17C
His19-Val197
Accession # Q9P0M4.1
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Fluorescein

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of IL‑17C in PC‑3 Human Cell Line by Flow Cytometry. View Larger

Detection of IL‑17C in PC‑3 Human Cell Line by Flow Cytometry. PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human IL‑17C Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1234F, filled histogram) or isotype control antibody (Catalog # IC003F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17C

The Interleukin-17 (IL-17) family proteins, comprising six members (IL-17/IL-17A through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus (1, 2). With the exception of IL-17B, which exists as a non‑covalently linked dimer, all IL‑17 family members are disulfide-linked dimers (3). IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2). Five receptors (IL-17 R, and IL-17RB through IL-17RE), which are likely activated by IL-17 family members, have been identified (1-4). Human IL-17C cDNA encodes a 197 amino acid (aa) residues protein with a putative 18 aa signal peptide (5). IL-17C shares from 15%‑30% aa sequence identity with other IL-17 family members. Human and mouse IL-17C also share 83% aa sequence identity. IL-17C has a very restricted expression pattern and was detected as a rare expressed sequence tag (EST) sequence in an adult prostate and fetal kidney libraries (2). As such, it is now known to be secreted by keratinocytes and colonic epithelium, and to act on epithelium via an IL-17RA:IL-17RE heterodimeric receptor. This is either an autocrine or paracrine activity (1,2,5). IL-17C has been shown to stimulate the release of proinflammatory cytokines and antimicrobial peptides such as S100A12, hBD, and CSF-3 (5, 6).

References
  1. Gu, C. et al. (2013) Cytokine 64:477.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Hymowitz, S.G. et al. (2001) EMBO J. 20:5332.
  4. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  5. Pappu, R. et al. (2012) Trends Immunol. 33:343.
  6. Kusagaya, H. et al. (2014) Am. J. Respir. 50:30.
Long Name
Interleukin 17C
Entrez Gene IDs
27189 (Human); 234836 (Mouse)
Alternate Names
CX2; Cytokine CX2; IL17C; IL-17C; IL-17CMGC126884; interleukin 17C; interleukin-17C; MGC138401

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