Detects human IL‑17E/IL‑25 in direct ELISAs and Western blots. Shows 100% cross-reactivity with recombinant mouse IL‑17E and no cross-reactivity with recombinant human (rh) IL-17, rhIL-17B, rhIL-17C, or rhIL-17F.
Monoclonal Mouse IgG1 Clone # 182203
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human IL‑17E/IL‑25 Tyr33-Gly177 Accession # Q9H293
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of IL‑17E/IL‑25 in PC‑3 human prostate cancer cell line by Flow Cytometry.
PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human IL‑17E/IL‑25 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC1258F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
The Interleukin 17 (IL-17) family proteins, comprising six members (IL-17, and IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).
Human IL-17E cDNA encodes a 177 amino acid (aa) residues precursor protein with a putative 32 aa signal peptide (3). A second isoform of human IL-17E encoding a 161 aa precursor protein also exists (4). The two isoforms differ in their signal peptide sequences. Mature human IL-17E shares 76% aa sequence identity with mature mouse IL-17E. Human IL-17E also shares from 25-36% aa sequence identity with the other human IL-17 family members. IL-17E expression was detected at very low levels by PCR in various peripheral tissues including brain, kidney, lung, prostate, testis, adrenal gland, spinal cord, and trachea (3). IL-17E binds and activates IL-17 B Receptor (IL-17B R) (alternatively known as IL-17 Rh1, IL-17E R, and EVI27) (3), which is expressed in kidney and liver, and at lower levels in brain, testis, and other endocrine tissues. The expression of IL-17B R is up regulated under inflammatory conditions. Ligation of IL-17E to IL-17 RB induces activation of nuclear factor kappa-B and stimulates the production of the pro-inflamatory cytokine IL-8 (3). IL-17 has also been found to promote the expression of the prototypical Th2 genes (4, 5).
Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
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