Intracellular Staining by Flow Cytometry
|Detection of IL‑17F in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes either (A) untreated or (B) treated with PMA and Calcium Ionomycin were stained with Mouse Anti-Human IL‑17F PE‑conjugated Monoclonal Antibody (Catalog # IC13351P) and Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.|
Interleukin-17F (IL-17F) is a disulfide-linked dimer belonging to the IL-17 family. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions.