Human IL-27/IL-35 EBI3 Subunit Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 607201
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit
    Arg21-Lys229 (predicted)
    Accession # Q14213
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑27/IL‑35 EBI3 Subunit in IL-18-treated KG‑1 Human Cell Line by Flow Cytometry. KG‑1 human acute myelogenous leukemia cell line, resting (open histogram) or treated with rhIL-18 (filled histogram) was stained with Mouse Anti-Human IL‑27/IL‑35 EBI3 Subunit Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6456G) or isotype control antibody (Catalog # IC002G). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-27/IL-35 EBI3 Subunit

IL-27 is a heterodimeric cytokine comprised of the IL-12 family that is composed of two chains, EBV-induced gene 3( EBI3), a 33 kDa glycosylated protein homologous to the p40 subunit of IL-12, and p28, a 28 kDa peptide analogous to the p35 subunit of IL-12. IL-27 has been found to be expressed by activated monocytes and monocyte-derived dendritic cells, astrocytes, adipocytes, CD4+ T cells, and macrophages. In human tissues, EBI3 and p28 coexpression has been observed in macrophages or macrophage-derived cells, and in endothelial cells. The IL-27 receptor complex comprises two chains, IL-27R (also called WSX-1 or TCCR) and gp130. IL-27 is the only known ligand for IL-27R, whereas gp130 is a receptor component of several cytokines of the IL-6 family. Functionally, IL-27 appears to be immunosuppressive. On T cells in mice, it induces IL-10 secretion while suppressing IL-17A and GM-CSF production. But the relationship between IL-27 and IL-17A may be complex. After IL-17A induction, IL-27 secretion occurs in unstimulated cells, suggesting IL-27 downregulates bystander activation in inflammatory conditions. EBI3 also complexes with p35 (IL-12) to form IL-35. The mature EBI3 sequences for human and mouse share 62% amino acid sequence identity.

  • Long Name:
    Epstein-Barr virus induced gene 3
  • Entrez Gene IDs:
    10148 (Human); 50498 (Mouse); 680609 (Rat)
  • Alternate Names:
    cytokine receptor; EBI3; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 subunit beta; IL27B; IL-27B; interleukin-27 subunit beta
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 Alexa Fluor® 488-conjugated Antibody

Ctrl IC002G 2
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 4
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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