Human IL‑27/IL‑35 EBI3 Subunit APC‑conjugated Antibody

1 citations
  • Specificity
    Detects human IL-27/IL-35 EBI3 Subunit in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) IL-27 EBI3 or rmIL-27 (EBI3 + p28 heterodimer) is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 607201
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human IL-27/IL-35 EBI3 Subunit
    Arg21-Lys229 (predicted)
    Accession # Q14213
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of IL‑27/IL‑35 EBI3 Subunit in KG‑1 Human Cell Line by Flow Cytometry. KG‑1 human acute myelogenous leukemia cell line was stained with Mouse Anti-Human IL‑27/IL‑35 EBI3 Subunit APC‑conjugated Monoclonal Antibody (Catalog # IC6456A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-27/IL-35 EBI3 Subunit

IL-27 is a heterodimeric cytokine comprised of the IL-12 p35-related protein, p28, and the IL-12 p40-related protein, EBI3 (Epstein-Barr virus-Induced gene 3). IL-27 is expressed by monocytes, endothelial cells and dendritic cells. It binds TCCR/WSX-1 on naive CD4+ T cells and induces the expression of a functional IL-12 receptor, making these cells sensitive to IL-12-mediated Th1 cell development. Human EBI3 also associates with the p35 subunit of IL-12 to form IL-35 which is important for immunosuppressive Treg cell induction. EBI3 is 61% amino acid (aa) identical to mouse EBI3 and includes an 20 aa signal peptide and a 209 aa mature protein with two fibronectin type III domains.

  • Long Name:
    Epstein-Barr virus induced gene 3
  • Alternate Names:
    EBI3; IL-27B; cytokine receptor; EBV-induced gene 3 protein; Epstein-Barr virus induced 3; Epstein-Barr virus induced gene 3; Epstein-Barr virus-induced gene 3 protein; IL-27 subunit beta; IL27B; IL-27B; interleukin-27 subunit beta
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 1 of 1

  1. IL-35 over-expression increases apoptosis sensitivity and suppresses cell growth in human cancer cells.
    Biochem Biophys Res Commun, 2013;430(1):364-9.
    Species: Human
    Sample Type: whole cells
    Application: Flow Cytometry,


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