Human IL-5 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant mouse IL-5 is observed and 15% cross-reactivity with recombinant rat IL-5 is observed. Neutralizes the biological activity of recombinant human IL-5 and will also neutralize the biological activity of recombinant mouse IL-5, although four times the amount of IgG is required.
  • Source
    Polyclonal Goat IgG
  • Purification
    Protein A or G purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-5
    Ile20-Ser134
    Accession # P05113
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Human IL‑5 (Catalog # 205-IL)
  • Neutralization
    Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 8-10 µg/mL in the presence of 1.25 ng/mL Recombinant Human IL‑5.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by IL‑5 and Neutralization by Human IL‑5 Antibody. Recombinant Human IL‑5 (Catalog # 205-IL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑5 (1.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑5 Polyclonal Antibody (Catalog # AB-205-NA). The ND50 is typically 8-10 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 1 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-5

Interleukin 5 is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 has also been shown to be a growth and differentiation factor for B cells. Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. The cDNAs for murine and human IL-5 encode precursor proteins with signal peptides that are cleaved to form mature proteins containing 113 and 115 amino acid residues, respectively. Murine and human IL-5 are 70% identical in their amino acid sequences and show species cross‑reactivity. The genes for human and mouse IL-5 have been mapped to chromosome 5 and chromosome 11, respectively; closely linked to the genes for IL-3, IL-4 and GM-CSF.

IL-5 exerts its activity on target cells by binding to specific cell surface receptors. The functional high‑affinity receptor for human IL-5 has been shown to be composed of a low-affinity IL-5 binding alpha -subunit and a non-binding common beta -subunit that is shared with the high‑affinity receptors for GM‑CSF and IL-3.

  • Long Name:
    Interleukin 5
  • Entrez Gene IDs:
    3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
  • Alternate Names:
    BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I
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