Human Indoleamine 2,3-dioxygenase/IDO Alexa Fluor® 700-conjugated Antibody

IC6030N has been discontinued and is replaced by IC6030AIC6030P.
  
  • Species Reactivity
    Human
  • Specificity
    Detects human Indoleamine 2,3-dioxygenase/IDO in direct ELISAs.
  • Source
    Monoclonal Mouse IgG1 Clone # 700838
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Indoleamine 2,3-dioxygenase/IDO
    Ala2-Gly403
    Accession # P14902
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of Indoleamine 2,3‑dioxygenase/IDO in Human MDSCs by Flow Cytometry. Human PBMC-derived myeloid-derived suppressor cells (MDSCs) treated with 10 ng/mL Recombinant Human IL‑6 (Catalog # 206-IL) and 10 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days were stained with Mouse Anti-Human Siglec‑3/CD33 PE‑conjugated Monoclonal Antibody (Catalog # FAB1137P) and either (A) Mouse Anti-Human Indoleamine 2,3‑dioxygenase/IDO Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # IC6030N) or (B) Mouse IgG1 PerCP Isotype Control (Catalog # IC002C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Indoleamine 2,3-dioxygenase/IDO
Indoleamine 2,3-dioxygenase (IDO) is a heme-containing intracellular dioxygenase catalyzing the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine (1). This degradation is the first and rate-limiting step of the L-kynurenine pathway (2). IDO is widely expressed in dendritic cells, macrophages, microglia, eosinophils, fibroblasts, endothelial cells, and most tumor cells. In immune cells, its expression is mainly induced by cytokines such as IFN-gamma, IFN-alpha, IFN-beta, and IL‑10. IDO has an antimicrobial function due to its decreasing the availability of the essential amino acid tryptophan in inflammatory environments (3). Recent studies have demonstrated that IDO induces immunosuppression during infection, pregnancy, transplantation, autoimmunity, and neoplasia (3-5).
  • References:
    1. Lewis-Ballester, A. et al. (2009) Proc. Natl. Acad. Sci. USA. 106:17371.
    2. Costantino, G. (2009) Expert Opin. Ther. Targets 13:247.
    3. Xu, H. et al. (2008) Immunol. Lett. 121:1.
    4. Lob, S. et al. (2009) Nat. Rev. Cancer 9:445.
    5. Curti, A. et al. (2009) Blood 113:2394.
  • Entrez Gene IDs:
    3620 (Human); 15930 (Mouse)
  • Alternate Names:
    3dioxygenase; EC 1.13.11.52; IDO; IDO1; IDOIDO-1; INDO; INDOindole 2,3-dioxygenase; Indoleamine 2; indoleamine 2,3-dioxygenase 1; Indoleamine 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; Indoleamine-pyrrole 2,3-dioxygenase
Related Research Areas

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Order Details

    IC6030N has been replaced by
    IC6030A
    IC6030P

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