Detection of Human iNOS aa 781-798 by Western Blot. Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # 201‑LB, 210‑TA, 285‑IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human iNOS Monoclonal Antibody (Catalog # MAB9502), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
iNOS in Human Brain. iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human iNOS aa 781-798 Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Inducible nitric oxide synthase (iNOS)
is a widely expressed, approximately 130 kDa enzyme that catalyzes the
conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a
bioactive mediator that plays an important role in hemodynamics by inducing
vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney.
In other tissues, it is inducibly expressed during inflammation, oxidative
stress, and hyperglycemia. iNOS activity is elevated in a variety of disease
states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute
kidney injury, TNF-alpha induced muscle wasting, and the increased
radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS
shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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