Detection of Integrin alpha 5/CD49e in K562 Human Cell Line by Flow Cytometry.
K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human Integrin alpha 5/CD49e Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB1864G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin alpha 5/CD49e
The Integrin alpha 5 subunit, also called CD49e, associates with the Integrin beta 1 subunit (CD29) to form the VLA-5 complex. It functions as a receptor for fibronectin and is expressed on fibroblasts, endothelial cells, epithelial cells, platelets and some melanoma cell lines (1, 2).
Springer, T.A. et al. (1982) Immunol. Rev. 68:171.
Te Velde, A. et al. (1988) J. Immunol. 140:1548.
Entrez Gene IDs:
3678 (Human); 16402 (Mouse)
CD49 antigen-like family member E; CD49e antigen; CD49e; Fibronectin receptor subunit alpha; fibronectin receptor, alpha subunit; FNRAVLA5A; Integrin alpha 5; integrin alpha-5; Integrin alpha-F; integrin, alpha 5 (fibronectin receptor, alpha polypeptide); ITGA5; very late activation protein 5, alpha subunit; VLA-5 alpha; VLA-5
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