Human Integrin  beta 2/CD18 APC-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Integrin beta 2/CD18 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Integrin  beta 1 or recombinant mouse Integrin  alpha 5 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 212701
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Integrin  beta 2/CD18
    Accession # AAA59490
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Integrin  beta 2/CD18 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human Integrin  beta 2/CD18 APC‑conjugated Monoclonal Antibody (Catalog # FAB1730A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Integrin beta 2/CD18

Integrin alpha X beta 2, also called CD11c/CD18, p150/95 or Complement Receptor type 4 (CR4), is one of four beta 2 integrins. The non-covalent heterodimer of 150 kDa alpha X/CD11c and 95 kDa beta 2/CD18 integrin subunits is expressed on macrophages, dendritic cells and hairy cell leukemias, with lower amounts on other myeloid cells and activated B, NK and some cytotoxic T cells (1-7). Like other integrins, alpha X beta 2 has multiple activation states (3). In the presence of divalent cations and "inside-out" signaling, alpha X beta 2 is fully active and extended. The alpha X vWFA or I-domain, which contains the adhesion sites, forms the N-terminal head region with the alpha X beta-propeller and the beta 2 vWFA domain (1, 8). In the inactive state, the heterodimer flexes in the center at the alpha X thigh and calf domains and beta 2 I-EGF domains, impeding access to adhesion sites (1). The 1088 amino acid (aa) human alpha X/CD11c ECD shares 70-76% aa sequence identity with mouse, rat and canine alpha X while the 678 aa human beta 2/CD18 ECD shares 81-83% aa sequence identity with mouse, rat, cow, dog, goat, sheep, and pig beta 2. Potential alpha X isoforms containing 719 and 725 aa (as compared to full-length 1163 aa alpha X) lack the vWFA domain and the N-terminus. Active alpha X beta 2 shares some adhesion partners with alpha M beta 2/CD11b/CD18, including complement opsonin fragment iC3b, ICAMs, vWF and fibrinogen, and is expressed on many of the same cells (4-11). However, alpha M beta 2 activity is often constitutive, while alpha X beta 2 activity requires cell activation (4-7). alpha X beta 2 also binds osteopontin, Thy-1, plasminogen, heparin, and proteins with abnormally exposed acidic residues (11-16). The adhesion events are important for proliferation, degranulation, chemotactic migration, and phagocytosis of complement-opsonized particles (5, 6, 9, 11, 12, 16). Mutations of beta 2, especially in the vWFA domain, cause Leukocyte Adhesion Deficiency (LAD-1) and susceptibility to bacterial infections (17).

  • References:
    1. Corbi, A.L. et al. (1987) EMBO J. 6:4023.
    2. Kishimoto, T.K. et al. (1987) Cell 48:681.
    3. Hynes, R.O. (2002) Cell 110:673.
    4. Arnaout, M.A. (1990) Blood 75:1037.
    5. Postigo, A.A. et al. (1991) J. Exp. Med. 174:1313.
    6. Beyer, M. et al. (2005) Respir. Res. 6:70.
    7. Nicolaou, F. et al. (2003) Blood 101:4033.
    8. Vorup-Jensen, T. et al. (2003) Proc. Natl. Acad. Sci. USA 100:1873.
    9. Bilsland, C.A.G. et al. (1994) J. Immunol. 152:4582.
    10. Pendu, R. et al. (2006) Blood 108:3746.
    11. Sadhu, C. et al. (2007) J. Leukoc. Biol. 81:1395.
    12. Schack, L. et al. (2009) J. Immunol. 182:6943.
    13. Choi, J. et al. (2005) Biochem. Biophys. Res. Commun. 331:557.
    14. Gang, J. et al. (2007) Mol. Cells 24:240.
    15. Vorup-Jensen, T. et al. (2007) J. Biol. Chem. 282:30869.
    16. Vorup-Jensen, T. et al. (2004) Proc. Natl. Acad. Sci. USA 102:1614.
    17. Kishimoto, T.K. et al. (1987) Cell 50:193.
  • Entrez Gene IDs:
    3689 (Human); 16414 (Mouse); 309684 (Rat)
  • Alternate Names:
    959 beta subunit precursor)10; CD18 antigen; CD18; CD18beta 2; cell surface adhesion glycoprotein (LFA-1; CR3; integrin beta-2; integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1;macrophage antigen 1 (mac-1) beta subunit); integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); ITGB2; LAD; LCAMB; leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C; LFA-1 beta; LFA-1; MAC-1 beta; MF17; MFI7; P150; p150,95 beta
Related Research Areas
Isotype Controls
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Mouse IgG1 APC-conjugated Antibody

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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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