Detection of LAIR2 in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human LAIR2 Fluorescein‑conjugated Monoclonal Antibody (Catalog # IC2665F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
LAIR2 (leukocyte-associated Ig-like receptor-2; also known as CD306) is a secreted, 131 amino acid (aa) protein that contains one Ig-like C2 type domain, making it a member of the Ig superfamily. When compared to LAIR1, its transmembrane counterpart, it shares 83% aa identity across the signal sequence and extracellular domains (1‑3). Although one is secreted and one is membrane-bound, the two LAIR proteins are thought to have arisen from a common gene ancestor and appear to share similar adhesion profiles. This suggests that LAIR2 may compete with LAIR1 for ligand binding (3, 4). A 114 aa alternate splice form of LAIR2 is truncated at the C terminus, but retains the entire Ig domain (1‑3). The expression profile of these splice forms, and the presence of orthologs in other species, have not been reported.
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